The imbalance of biliary microflora in hepatolithiasis
The imbalance of biliary microflora in hepatolithiasis
复制标题
肝内胆管结石病胆道微生物群的失衡
DOI：
10.1016/j.micpath.2021.104966
复制
发表时间：
2021-05-31
影响因子：
3.8
通讯作者：
Wu, LinQuan
中科院分区：
文献类型：
--
作者：
Xiao, ZhouQing;Huang, ZhiHao;Wu, LinQuan
背景：微生物区系失衡被认为与许多疾病有关。然而，胆道微生物区系的特点及其与肝内胆管结石的关系尚不清楚。方法：本研究包括40例肝内胆管结石患者和10例对照组。在肝切除手术中采集胆汁样本，进行16S rRNA测序。对测序结果进行操作分类单元(OTU)聚类、物种注释和丰度分析、样本复杂性分析、多样性分析和环境因素相关分析。结果：肝胆管结石组与对照组胆汁微生物区系有显著差异。我们发现肝内胆管结石患者胆汁微生物丰度较高(52.4%比40.2%，42.1%比29.6%)。肝胆管结石患者胆汁微生物区系多样性显著降低(香农(P=0.004)、观察种(P=0.001)、全树(P=0.001))。这些差异主要与肠球菌有关(P
Background: The imbalance of microbial flora is thought to be associated with many diseases. However, the characteristics of the biliary microflora and its relation to in hepatolithiasis are unknown.Methods: This study included 40 patients with hepatolithiasis and 10 control patients. Bile samples were taken during hepatectomy surgeries and 16S rRNA sequencing was performed. The sequencing results were analyzed by operational taxonomic unit (OTU) clustering, species annotation and abundance analyses, sample complexity analyses, diversity analyses, and environmental factor correlation analyses.Results: There were significant differences in bile microflora between the hepatolithiasis group and the control group. We found that the abundance of microflora in the bile of patients with hepatolithiasis was relatively high (52.4% versus 40.2% and 42.1% versus 29.6%). The diversity of microflora in the bile of patients with hep-atolithiasis decreased significantly (Shannon (P = 0.004), Observed species (P = 0.001), PD-whole-tree (P = 0.001)). These differences are mainly associated with Enterococcus(P