DNA-SEQUENCES COMPLEMENTARY TO HUMAN 7-SK RNA SHOW STRUCTURAL SIMILARITIES TO THE SHORT MOBILE ELEMENTS OF THE MAMMALIAN GENOME
DNA-SEQUENCES COMPLEMENTARY TO HUMAN 7-SK RNA SHOW STRUCTURAL SIMILARITIES TO THE SHORT MOBILE ELEMENTS OF THE MAMMALIAN GENOME
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DOI：
10.1016/0022-2836(84)90038-x
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发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
MELLI, M
中科院分区：
文献类型：
--
作者：
MURPHY, S;ALTRUDA, F;MELLI, M
从人宫颈癌HeLa细胞中克隆了7个SK RNA的互补DNA克隆，用于研究人类基因组中7个SK序列的基因组结构。基因组杂交和基因组克隆表明，7 SK与一个分散的重复序列家族同源，其中大多数缺少7 SK RNA序列的3“末端。只有少数基因组K序列与3“和5”7 SK探针同源，并可能包括7 SK RNA的基因。4个基因组7 SK克隆的序列证实它们在大多数情况下是假基因。虽然Alu序列经常被发现在K DNA的3“和5”末端附近，但在所研究的所有克隆中，紧邻假基因侧翼的序列是不同的。在KDNA或K-Alu单元的直接侧翼发现了直接重复序列，这表明K序列单独或与Alu DNA一起可能构成移动的元件。
A complementary DNA clone of 7 SK RNA from HeLa [human cervical carcinoma] cells was used to study the genomic organization of 7 SK sequences in the human genome. Genomic hybridization and genomic clones show that 7 SK is homologous to a family of disperse repeated sequences most of which lack the 3'' end of the 7 SK RNA sequence. Only few of the genomic K sequences are homologous to both 3'' and 5'' 7 SK probes and presumably include the gene(s) for 7 SK RNA. The sequence of 4 genomic 7 SK clones confirms that they are in most cases pseudogenes. Although Alu sequences are frequently found near the 3'' and 5'' end of K DNA, the sequences immediately flanking the pseudogenes are different in all clones studied. Direct repeats were found flanking directly the K DNA or the K-Alu unit, suggesting that the K sequences alone or in conjunction with Alu DNA might constitute a mobile element.