Localization of specific binding sites for bombesin in the canine gastrointestinal tract.
Localization of specific binding sites for bombesin in the canine gastrointestinal tract.
复制标题
犬胃肠道中铃蟾肽特异性结合位点的定位。
DOI：
10.1016/0016-5085(87)90257-5
复制
发表时间：
1987
期刊：
影响因子：
29.4
通讯作者：
Mantyh,PW
中科院分区：
文献类型：
--
作者：
Vigna,SR;Mantyh,CR;Giraud,AS;Soll,AH;Walsh,JH;Mantyh,PW
这些研究的目的是确定犬胃肠道中具有蛙皮素/胃泌素释放肽特异性结合位点的组织和细胞类型。将单碘化的生物活性（Tyr-4）-蛙皮素14（100 pM）应用于犬肠道切片，并使用定量放射自显影术进行定位。最高密度的蛙皮素/胃泌素释放肽特异性结合位点发生在胃窦粘膜内分泌细胞。在胃底、胃窦和回肠的环形肌层上，在胃底和胃窦的纵肌上，以及在胃底、胃窦和小肠的肌间神经丛中的神经元上也发现了特异性结合位点。在犬食管、贲门、胆囊、胰腺或结肠切片中未发现125 I-（Tyr-4）-蛙皮素14特异性结合的证据。这些结果表明，直接作用的蛙皮素和内源性胃泌素释放肽的胃泌素释放和胃肠动力的网站。
The goal of these studies was to determine the tissue and cell types possessing specific binding sites for bombesin/gastrin-releasing peptide in the canine gastrointestinal tract. Monoiodinated, biologically active (Tyr-4)-bombesin 14 (100 pM) was applied to sections of canine gut and localized using quantitative autoradiography. The highest density of bombesin/gastrin-releasing peptide specific binding sites occurred over endocrine cells in the antral mucosa. Specific binding sites were also found on the circular muscle layer of the gastric fundus, gastric antrum, and ileum, on longitudinal muscle of the gastric fundus and antrum, and on neuronal elements in the myenteric plexus in the gastric fundus, antrum, and small intestine. No evidence for specific binding of125I-(Tyr-4)-bombesin 14 was found in sections of canine esophagus, gastric cardia, gallbladder, pancreas, or colon. These results suggest sites of direct action of bombesin and endogenous gastrin-releasing peptide for gastrin release and gastrointestinal motility.