Multidrug-Resistant Salmonella Isolates from Retail Chicken Meat Compared with Human Clinical Isolates
Multidrug-Resistant Salmonella Isolates from Retail Chicken Meat Compared with Human Clinical Isolates
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DOI：
10.1089/fpd.2009.0499
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
McDermott, Patrick F.
中科院分区：
文献类型：
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作者：
M'ikanatha, Nkuchia M.;Sandt, Carol H.;McDermott, Patrick F.
目的：为了研究鸡肉中的抗生素耐药沙门氏菌的患病率，并与患病human.Methods分离：我们从随机选择的零售店在宾夕法尼亚州中部在2006-2007年期间购买的生鸡肉分离沙门氏菌。沙门氏菌从肉类分离进行了比较，使用脉冲场凝胶电泳，在PulseNet数据库中的沙门氏菌从human.Results恢复的分离株：378鸡肉样品，84（22%）含有沙门氏菌。26株（31%）沙门氏菌分离株对≥ 3种抗菌剂耐药，18株（21%）对头孢噻呋耐药。通过聚合酶链反应检测，所有头孢噻呋耐药分离株对头孢曲松的敏感性降低（最小抑菌浓度>2 mg/mu L），并携带blaCMY基因。在28株鼠伤寒沙门氏菌分离株中，20株（71.4%）对≥ 3种抗菌药物耐药，12株（42.9%）对头孢噻呋耐药。在PulseNet中，一种头孢噻呋耐药鼠伤寒沙门氏菌家禽分离株显示出与人类分离株难以区分的罕见脉冲场凝胶电泳图谱;两种分离株均携带bla（CMY-2）基因。这些数据表明禽肉中存在多重耐药沙门氏菌，包括blaCMY质粒介导的基因，该基因赋予对家禽中使用的头孢噻呋和头孢曲松的耐药性，用于治疗人类沙门氏菌病。这项研究说明了分子分型数据库的潜力，以确定相关的沙门氏菌分离的肉类和生病的人，并表明，鸡可能是一个来源的多重耐药沙门氏菌病在人类。
Aim: To examine the prevalence of antimicrobial-resistant Salmonella in chicken meat and correlate with isolates from ill humans.Methods: We isolated Salmonella from raw chicken purchased from a randomly selected sample of retail outlets in central Pennsylvania during 2006-2007. Salmonella isolates from meat were compared, using pulsed-field gel electrophoresis, to isolates in the PulseNet database of Salmonella recovered from humans.Results: Of 378 chicken meat samples, 84 (22%) contained Salmonella. Twenty-six (31%) of the Salmonella isolates were resistant to >= 3 antimicrobials and 18 (21%) were resistant to ceftiofur. All ceftiofur-resistant isolates exhibited reduced susceptibility (minimum inhibitory concentration >2 mg/mu L) to ceftriaxone and carried a blaCMY gene, as detected by polymerase chain reaction. Among the 28 Salmonella serovar Typhimurium isolates, 20 (71.4%) were resistant to >= 3 antimicrobials and 12 (42.9%) were resistant to ceftiofur. One ceftiofur-resistant Salmonella serovar Typhimurium poultry isolate exhibited a rare pulsed-field gel electrophoresis pattern indistinguishable from a human isolate in PulseNet; both isolates carried the bla(CMY-2) gene.Conclusions: These data demonstrate the presence of multidrug-resistant Salmonella in poultry meat, including blaCMY plasmid-mediated genes that confer resistance to both ceftiofur, used in poultry, and ceftriaxone, used for treating salmonellosis in humans. This study illustrates the potential for molecular subtyping databases to identify related Salmonella isolates from meat and ill humans, and suggests that chicken could be a source for multidrug-resistant salmonellosis in humans.