THE DEVELOPMENT EXPRESSION OF THE RAT ALPHA-VASCULAR AND GAMMA-ENTERIC SMOOTH-MUSCLE ISOACTINS - ISOLATION AND CHARACTERIZATION OF A RAT GAMMA-ENTERIC ACTIN CDNA
THE DEVELOPMENT EXPRESSION OF THE RAT ALPHA-VASCULAR AND GAMMA-ENTERIC SMOOTH-MUSCLE ISOACTINS - ISOLATION AND CHARACTERIZATION OF A RAT GAMMA-ENTERIC ACTIN CDNA
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DOI：
10.1128/mcb.8.12.5224
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发表时间：
1988-12-01
影响因子：
5.3
通讯作者：
LESSARD, JL
中科院分区：
文献类型：
--
作者：
MCHUGH, KM;LESSARD, JL
我们已经从整个大鼠胃中分离并鉴定了两个cDNA克隆，pRV α A-19和pRE γ A-11，它们对α-SMA具有特异性。血管和γ-肠平滑肌异肌动蛋白。老鼠γ-肠平滑肌肌动蛋白与鸡的γ-肌砂囊相比，在成熟肽的第359位含有脯氨酸对谷氨酰胺的单一氨基酸取代。肌动蛋白序列（J. Vandekerckhove和K. Weber，FEBS Lett. 102：219，1979）。两种平滑肌肌动蛋白cDNA的5“和3”非翻译（UT）区的序列比较表明，这些区域不包含明显的序列相似性。两种平滑肌肌动蛋白cDNA的5 ″ UT区与所有其它已知的肌动蛋白序列的另外比较显示，大鼠γ-肌动蛋白的序列没有明显的相似性。肠异肌动蛋白在目前可获得的序列的15个碱基对内，而大鼠α-血管异肌动蛋白含有两个独立的序列，它们与人和鸡α-肌动蛋白的5 ″ UT区域内的序列相似。血管肌动蛋白基因。两种平滑肌肌动蛋白的3“UT区域的类似比较表明，α-肌动蛋白的3”UT区域与α-肌动蛋白的3“UT区域的相似性。血管异肌动蛋白不含有对其它异肌动蛋白观察到的高度跨物种序列保守性，肠异肌动蛋白含有52个核苷酸的反向序列，其与人、鸡和大鼠β的3”UT区域内发现的序列相似胞质异肌动蛋白这些观察复杂的明显跨物种的保护，这些领域的同种型特异性先前观察到的其他肌动蛋白亚型。北方印迹分析的第15天大鼠胚胎和新生儿，出生后第19天，和成年大鼠表明，第15天大鼠胚胎显示低至不可检测水平的平滑肌异肌动蛋白mRNA表达。出生时，胃和小肠中α-谷氨酰胺显著增加。血管和γ-肠肌动蛋白表达。这些最初的高表达水平在第19天至成年期下降。在成年大鼠中，子宫和主动脉平滑肌isoactin mRNA的含量不同。这些结果表明，γ-肠和α-血管异肌动蛋白mRNA在含有平滑肌的组织中以不同程度共表达。
We have isolated and characterized two cDNA clones from whole rat stomach, pRV.alpha.A-19 and pRE.gamma.A-11, which are specific for the .alpha.-vascular and .gamma.-enteric smooth muscle isoactins, respectively. The rat .gamma.-enteric smooth muscle actin contains a single amino acid substitution of a proline for a glutamine at position 359 of the mature peptide when compared with the chicken gizzard .gamma.-actin sequence (J. Vandekerckhove and K. Weber, FEBS Lett. 102: 219, 1979). Sequence comparisons of the 5'' and 3'' untranslated (UT) regions of the two smooth muscle actin cDNAs demonstrate that these regions contain no apparent sequence similarities. Additional comparisons of the 5'' UT regions of the two smooth muscle actin cDNAs to all other known actin sequences reveal no apparent sequence similarities for the rat .gamma.-enteric isoactin within the 15 base pairs of sequence currently available, while the rat .alpha.-vascular isoactin contains two separate sequences which are similar to sequences within the 5'' UT regions of the human and chicken .alpha.-vascular actin genes. A similar comparison of the 3'' UT regions of the two smooth muscle actins demonstrates that the .alpha.-vascular isoactins do not contain the high degree of cross-species sequence conservation observed for the other isoactins and that the .gamma.-enteric isoactin contains an inverted sequence of 52 nucleotides which is similar to a sequence found within the 3'' UT regions of the human, chicken, and rat .beta.-cytoplasmic isoactins. These observations complicate the apparent cross-species conservation of isotype specificity of these domains previously observed for the other actin isoforms. Northern blot analysis of day 15 rat embryos and newborn, day 19 postbirth, and adult rats demonstrates that the day 15 rat embryo displays low to undetectable levels of smooth muscle isoactin mRNA expression. By birth, the stomach and small intestine show dramatic increases in .alpha.-vascular and .gamma.-enteric actin expression. These initially high levels of expression decrease through day 19 to adulthood. In the adult rat, the uterus and aorta differ in their content of smooth muscle isoactin mRNA. These results demonstrate that the .gamma.-enteric and .alpha.-vascular isoactin mRNAs are coexpressed to various degrees in tissues which contain smooth muscle.