CHANGES IN ELECTRICAL-PROPERTIES OF RAT MYOMETRIUM DURING GESTATION AND FOLLOWING HORMONAL TREATMENTS
CHANGES IN ELECTRICAL-PROPERTIES OF RAT MYOMETRIUM DURING GESTATION AND FOLLOWING HORMONAL TREATMENTS
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DOI：
10.1113/jphysiol.1976.sp011517
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发表时间：
1976-01-01
影响因子：
5.5
通讯作者：
SUZUKI, H
中科院分区：
文献类型：
--
作者：
KURIYAMA, H;SUZUKI, H
用微电极技术研究了妊娠期和卵巢激素处理后大鼠子宫肌层的膜特性。记录了非怀孕、怀孕和产后子宫肌层自发产生的与静默期交替的电活动爆发。膜电位在妊娠中期最高，但峰电位幅度不均匀。在妊娠末期和分娩期，膜电位较低，爆发性放电的峰电位频率较低，幅度均匀。在分娩和产后期间，膜的逐渐去极化，伴随着膜电阻的增加，发生在产生爆发之前。膜的兴奋性从爆发产生前的峰值波动到爆发停止后的低值。位移的膜电位通过电流或降低温度修改的斜率缓慢的自发去极化，但兴奋性的波动持续存在。自发爆发频率的Q10值，在36 - 30 ° C之间测量。C是3.8。膜的超极化增加了尖峰的最大上升速率，但超过-70 mV，上升速率降低。半失活的峰生成发生在膜电位小于负比爆发间电位，表明电流携带系统在分娩过程中没有完全激活。在正常和去势大鼠，雌二醇超极化的膜和突发的尖峰产生持续去极化。预后轻微超极化的膜和爆发式放电发生没有持续的去极化。同时用孕酮和雌二醇处理，在爆发性放电过程中产生了持续时间长的平台电位。肌肉层的厚度，长度常数的组织和时间常数的膜测量在妊娠期间和从绝育大鼠在各种激素条件下。雌二醇增加了组织的长度常数，并进一步增加了雌二醇和孕酮同时治疗。组织厚度的增加对长度常数的影响最为显著.孕酮处理的子宫肌层的静息和活性膜特性与妊娠中期观察到的相似。雌二醇处理的子宫肌层不像在妊娠和分娩的最后阶段，这是由2种激素，雌二醇前孕酮的组合模拟。
The membrane properties of the rat myometrium, during gestation and following ovarian hormone treatment, were investigated with the microelectrode technique. Spontaneously generated bursts of electrical activity alternating with silent periods were recorded from non-pregnant, pregnant and postpartum myometria. The membrane potential was highest during the middle stage of gestation, but the spike amplitude within a burst was not uniform. In the final stage of gestation and during parturition, the membrane potential was low and the spikes within a burst were of low frequency and uniform amplitude. During parturition and postpartum, a gradual depolarization of the membrane, accompanied by an increase in membrane resistance, occurred before the generation of a burst. Excitability of the membrane fluctuated from a peak just before the generation of a burst to a low after the cessation of a burst. Displacement of the membrane potential by electrical current or by lowering the temperature modified the slope of the slow spontaneous depolarization, but the fluctuations of excitability persisted. The Q10 value for the frequency of spontaneous bursts, measured between 36-30.degree. C, was 3.8. Hyperpolarization of the membrane increased the maximum rate of rise of the spike, but beyond -70 mV, the rate of rise was reduced. Half-inactivation of spike generation occurred at a membrane potential less negative than the interburst potential, indicating that the current carrying system was not fully activated during parturition. In both normal and spayed rats, estradiol hyperpolarized the membrane and the burst of spikes was generated on a sustained depolarization. Progesterone slightly hyperpolarized the membrane and burst discharges occurred without a sustained depolarization. Simultaneous treatment with progesterone and estradiol produced a plateau potential of long duration during burst discharges. The thickness of the muscle layer, length constant of the tissue and time constant of the membrane were measured during gestation and from spayed rats under various hormonal conditions. The length constant of the tissue was increased by estradiol and was further increased by simultaneous treatment with estradiol and progesterone. The increase in tissue thickness to have the most marked influence on the length constant. The resting and active membrane properties of the progesterone treated myometrium were similar to those observed during the middle stages of gestation. The estradiol-treated myometrium did not resemble that during the last stages of gestation and parturition, which was simulated by combination of the 2 hormones, estradiol preceding progesterone.