Collagen‐binding heat shock protein (HSP) 47 expression in anti‐thymocyte serum (ATS)‐induced glomerulonephritis
Collagen‐binding heat shock protein (HSP) 47 expression in anti‐thymocyte serum (ATS)‐induced glomerulonephritis
复制标题
抗胸腺细胞血清 (ATS) 诱导的肾小球肾炎中胶原结合热休克蛋白 (HSP) 47 的表达
作者：
M. Razzaque;T. Taguchi
细胞外基质（ECM）（主要是胶原）的积累增加是抗胸腺细胞血清（ATS）诱导的肾小球肾炎（GN）中扩张的系膜基质的主要成分。热休克蛋白（HSP）47是一种胶原结合应激蛋白，已被证明在前胶原分子的细胞内加工中具有特定作用。它是各种器官中的胶原蛋白特异性分子伴侣，但其在肾脏中与基质扩张相关的作用尚不清楚。本研究旨在评估ATS诱导的GN中ECM积累增加是否与HSP 47相关。在ATS诱导的GN大鼠肾脏中研究了I型、III型和IV型胶原及其分子伴侣HSP 47的表达。将15只雄性Wistar大鼠分为两组：ATS诱导的GN大鼠（I组）和年龄匹配的对照组（II组）。用ATS（0.8ml/100 g体重）诱发GN。于实验第3、10天处死大鼠。在组I中，ATS注射后3天，组织学检查显示肾小球细胞数量减少伴系膜溶解。然而，10天后，ATS注射，组织学上严重的系膜细胞增殖与系膜基质的扩张，注意到在组I大鼠。通过半定量分析，与对照组相比，第10天在ATS诱导的GN（I组）大鼠的扩张系膜基质中观察到I型、III型和IV型胶原免疫染色增加。免疫反应性HSP 47表达在肾小球内细胞中较弱，偶尔见于对照肾的间质细胞。相比之下，在第10天，在ATS处理的大鼠肾脏的肾小球中观察到强烈的HSP 47免疫染色。在本研究中，ATS处理的大鼠肾脏中各种胶原及其分子伴侣HSP 47平行增加。与对照组相比，在没有系膜基质扩张的ATS处理的大鼠肾脏中（ATS注射后3天），未发现HSP 47表达的显著差异。可以得出结论，HSP 47的过表达可能在胶原的过度组装中起重要作用，并且随后可能有助于ATS处理的大鼠肾脏中发现的系膜基质的扩张。© 1997年由John Wiley & Sons，Ltd.
An increased accumulation of extracellular matrix (ECM), predominantly collagens, is the main component of the expanded mesangial matrix in anti‐thymocyte serum (ATS)‐induced glomerulonephritis (GN). Heat shock protein (HSP) 47 is a collagen‐binding stress protein and has been shown to have a specific role in the intracellular processing of procollagen molecules. It is a collagen‐specific molecular chaperone in various organs, but its role in the kidney in relation to matrix expansion is not yet known. This study was designed to assess whether increased ECM accumulation in ATS‐induced GN is associated with HSP47. The expression of type I, type III and type IV collagens, with their molecular chaperone HSP47, was investigated in ATS‐induced GN rat kidneys. Fifteen male Wistar rats were divided into two groups: ATS‐induced GN rats (group I) and age‐matched controls (group II). GN was induced by injecting a single dose of ATS (0·8 ml/100 g body weight). All the rats were killed on the third and tenth day of the experiment. In group I, 3 days after ATS injection, histological examination revealed a reduction in glomerular cell number with mesangiolysis. However, 10 days after ATS injection, histologically severe mesangial cell proliferation with expansion of the mesangial matrix was noted in group I rats. By semiquantitative analysis, compared with controls, increased type I, type III, and type IV collagen immunostaining was observed in the expanded mesangial matrix in ATS‐induced GN (group I) rats on day 10. Immunoreactive HSP47 expression was weak in the intraglomerular cells and was occasionally seen in the interstitial cells in control kidneys. In contrast, strong immunostaining for HSP47 was noted in the glomeruli of the ATS‐treated rat kidneys on day 10. In this study, there was a parallel increase of various collagens and their molecular chaperone HSP47 in the ATS‐treated rat kidneys. Compared with controls, no significant difference in HSP47 expression was found in the ATS‐treated rat kidneys without mesangial matrix expansion (3 days after ATS injection). It is concluded that overexpression of HSP47 might play a significant role in the excessive assembly of collagens and could subsequently contribute to the expansion of mesangial matrix found in ATS‐treated rat kidneys. © 1997 by John Wiley & Sons, Ltd.
影响因子：
4.4
作者：
T. Yamamoto;C. Wilson
通讯作者：
T. Yamamoto;C. Wilson
DOI：
10.1016/s0021-9258(17)41724-8
发表时间：
1994-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Sauk;T. Smith;K. Norris;L. Ferreira
通讯作者：
J. Sauk;T. Smith;K. Norris;L. Ferreira
影响因子：
19.6
作者：
Tadashi Yamamoto;Curtis B. Wilson
通讯作者：
Tadashi Yamamoto;Curtis B. Wilson