Cryptosporidium tyzzeri n. sp (Apicomplexa: Cryptosporidiidae) in domestic mice (Mus musculus)
Cryptosporidium tyzzeri n. sp (Apicomplexa: Cryptosporidiidae) in domestic mice (Mus musculus)
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DOI：
10.1016/j.exppara.2011.07.012
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发表时间：
2012-03-01
影响因子：
2.1
通讯作者：
Xiao, Lihua
中科院分区：
文献类型：
--
作者：
Ren, Xupeng;Zhao, Jinfeng;Xiao, Lihua
家鼠小肠内的隐孢子虫最初被描述为隐孢子虫。隐孢子虫分离株的最新遗传学和生物学特征表明，家鼠感染了几种形态上难以区分的肠道隐孢子虫寄生虫，具有不同的宿主特异性，包括C。严格意义上的parvum，小鼠基因型I。和小鼠基因型II。在这项研究中，隐孢子虫小鼠基因型I的形态，生物学和遗传学特征进行了描述。作为C. parvum是1985年从小牛和人类分离的，命名为C.小隐孢子虫（Cryptosporidiumtyzzeri）已被广泛用于感染反刍动物和人类的寄生虫，小鼠基因型I被命名为隐孢子虫（Cryptosporidiumtyzzeri）。新种的卵囊（4.64 ± 0.05 μ m × 4.19 ± 0.06 μ m，平均形状指数1.11 ± 0.02; n = 69）比重新描述的C.小的新生小鼠和成年小鼠的开放前期分别为6天和7天，开放期分别为24-28天和28-29天。卵囊对羔羊和犊牛无感染性。光，透射电子显微镜和扫描电子显微镜的新物种的研究表明，在实验感染小鼠的空肠和回肠的微绒毛刷状缘的发育阶段的存在下，在回肠的感染最密集。其核苷酸序列与C.小孢子虫小亚基rRNA、70 kDa热休克蛋白、卵囊壁蛋白、肌动蛋白和60 kDa糖蛋白基因。基于形态学、遗传学和生物学数据，并符合已建立的隐孢子虫物种命名标准，这种地理上广泛分布的寄生虫被命名为一个新物种，以荣誉泰泽（Ernest Edward Tyzzer）的名字命名，泰泽是隐孢子虫研究的先驱。(C)2011 Elsevier Inc. All rights reserved.
The Cryptosporidium in the small intestine of domestic mice (Mus musculus) was initially described as Cryptosporidium parvum. Recent genetic and biologic characterization of Cryptosporidium isolates indicate that domestic mice are infected with several morphologically indistinguishable intestinal Cryptosporidium parasites with different host specificities, including C. parvum sensu stricto, mouse genotype I. and mouse genotype II. In this study, the morphological, biological, and genetic characteristics of the Cryptosporidium mouse genotype I are described. As a full re-description of C. parvum was made in 1985 for isolates from calves and humans and the name C. parvum has been widely used for the parasite that is infectious to both ruminants and humans, the mouse genotype I is named as Cryptosporidium tyzzeri. Oocysts of the new species (4.64 +/- 0.05 mu m x 4.19 +/- 0.06 mu m, with a mean shape index of 1.11 +/- 0.02; n = 69) are slightly smaller than those of the re-described C. parvum. The prepatent period was six and seven days, and the patent period was 24-28 and 28-29 days in neonatal and adult mice, respectively. Oocysts were not infectious to lambs and calves. Light, transmission electron and scanning electron microscopy studies of the new species showed the presence of developmental stages in the microvillar brush border of the jejunum and ileum of experimentally infected mice, with the infection most intensive in the ileum. It had nucleotide sequences significantly different from C. parvum at the small subunit rRNA, 70 kDa heat shock protein, oocyst wall protein, actin, and the 60 kDa glycoprotein genes. Based on the morphological, genetic, and biological data and in compliance of established Cryptosporidium species naming criteria, this geographically widespread parasite is named as a new species in honor of Ernest Edward Tyzzer, who pioneered Cryptosporidium research. (C) 2011 Elsevier Inc. All rights reserved.