A Network Pharmacology-Based Study on the Hepatoprotective Effect of Fructus Schisandrae.
A Network Pharmacology-Based Study on the Hepatoprotective Effect of Fructus Schisandrae.
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基于网络药理学的五味子保肝作用研究
DOI：
10.3390/molecules22101617
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发表时间：
2017-09-28
期刊：
影响因子：
--
通讯作者：
Feng Y
中科院分区：
文献类型：
--
作者：
Hong M;Zhang Y;Li S;Tan HY;Wang N;Mu S;Hao X;Feng Y
五味子是一种常见的中草药，用于治疗慢性肝病已有数百年的历史。五味子的疗效也在临床实践中得到了验证。本研究通过分子对接和网络分析，探讨五味子作为治疗肝病的有效途径的保肝作用机制。五味子的多种活性成分与44个与病毒性肝炎、脂肪肝、肝纤维化、肝硬变和肝癌相关的蛋白质靶点对接。通过网络药理分析，构建化合物-靶点网络，预测有效成分与靶点的关系。结果表明，五味子乙素、五味子乙素、五味子醇甲、五味子乙素、五味子素C、五味子乙素A、五味子乙素G和当归甲素可能与21种与肝脏疾病，特别是与脂肪性肝病相关的细胞内蛋白发生作用。五味子的保肝作用可能与细胞色素P2E1PPARα和AMPK基因及其相关途径有关。所使用的网络药理策略为寻找传统中草药和新型生物活性成分的作用机理提供了有力的工具。
Fructus schisandrae (Wuweizi in Chinese), a common traditional Chinese herbal medicine, has been used for centuries to treat chronic liver disease. The therapeutic efficacy of Wuweizi has also been validated in clinical practice. In this study, molecular docking and network analysis were carried out to explore the hepatoprotective mechanism of Wuweizi as an effective therapeutic approach to treat liver disease. Multiple active compounds of Wuweizi were docked with 44 protein targets related with viral hepatitis, fatty liver, liver fibrosis, cirrhosis, and liver cancer. A compound–target network was constructed through network pharmacology analysis, predicting the relationships of active ingredients to the targets. Our results demonstrated that schisantherin, schisandrin B, schisandrol B, kadsurin, Wuweizisu C, Gomisin A, Gomisin G, and angeloylgomisin may target with 21 intracellular proteins associated with liver diseases, especially with fatty liver disease. The CYP2E1, PPARα, and AMPK genes and their related pathway may play a pivotal role in the hepatoprotective effects of Wuweizi. The network pharmacology strategy used provides a forceful tool for searching the action mechanism of traditional herbal medicines and novel bioactive ingredients.