Enzyme Microelectrodes for Choline and Acetylcholine and Their Applications.
Enzyme Microelectrodes for Choline and Acetylcholine and Their Applications.
复制标题
胆碱和乙酰胆碱酶微电极及其应用。
DOI：
10.1016/0003-2670(94)00318-1
复制
发表时间：
1994
影响因子：
4.7
通讯作者：
Masashi
中科院分区：
文献类型：
--
作者：
Kano;Kenji;Morikage;Kazuo;Uno;Bunji;Esaka;Yukihiro;Masashi
通过使用戊二醛蒸气将乙酰胆碱酯酶 (AchE) 和胆碱氧化酶 (ChO) 共固定在铂盘微电极上，开发了基于过氧化氢的电流微传感器，用于胆碱 (Ch) 和乙酰胆碱 (Ach)。该传感器应用于微流量注射和微液相色谱分析。色谱峰高与 Ach 和 Ch 的含量在 0.05 至 103 pmol（8 × 10−7 至 1.5 × 10−2M，0.06 μl）范围内呈线性正比，相关系数 > 0.99。 ChO 的固定化用于 Ch 的选择性测定，响应时间为 5 s。该电极用于在总体积为 5.0 ml 的批量方法中测量 1 µl 体积中低至 0.25 U ml−1 的 AchE 活性。
Hydrogen peroxide based amperometric microsensors have been developed for choline (Ch) and acetylcholine (Ach) by coimmobilization of acetylcholinesterase (AchE) and choline oxidase (ChO) on platinum disk microelectrodes using glutaraldehyde vapor. The sensor was applied in micro flow injection and micro liquid chromatographic analyses. The chromatographic peak heights were linearly proportional to the amounts of Ach and Ch over the range of 0.05 to 103pmol (8 × 10−7to 1.5 × 10−2M, 0.06 μl) with correlation coefficients > 0.99. Immobilization of ChO was used for the selective determination of Ch with a response time of 5 s. This electrode was used for activity measurements of AchE down to 0.25 U ml−1in a 1 μl volume in a batch method at a total volume of 5.0 ml.
影响因子：
7.4
作者：
KRISTENSEN, EW;KUHR, WG;WIGHTMAN, RM
通讯作者：
WIGHTMAN, RM
影响因子：
7.4
作者：
BINDRA, DS;ZHANG, YN;REACH, G
通讯作者：
REACH, G