In vitro effect of over-the-counter probiotics on the ability of Candida albicans to form biofilm on denture strips.
In vitro effect of over-the-counter probiotics on the ability of Candida albicans to form biofilm on denture strips.
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2014-06
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通讯作者：
S. Ujaoney;J. Chandra;F. Faddoul;Maya Chane;Jing Wang;Louay Taifour;M. Mamtani;T. Thakre;H. Kulkarni;P. Mukherjee;M. Ghannoum
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作者：
S. Ujaoney;J. Chandra;F. Faddoul;Maya Chane;Jing Wang;Louay Taifour;M. Mamtani;T. Thakre;H. Kulkarni;P. Mukherjee;M. Ghannoum
益生菌在治疗和预防与义齿使用相关的口腔念珠菌病方面的潜力正受到越来越多的关注。我们的目的是调查市售的非处方益生菌是否可以影响白色念珠菌形成生物膜的能力，这被认为是口腔念珠菌病开始和进展的标志。方法采用2×5析因的体外实验方法，对培养的C.在义齿条上接种白色念珠菌，并用以下四种市售益生菌中的一种以细菌或无细胞上清液形式进行攻击：Accuflora®、Align®、Culturelle®和Sustenex®。C.在研究的所有因子组合中一式三份地研究白色念珠菌生物膜形成，并用荧光显微镜定性评估和用四唑盐（XTT）还原测定定量评估。质量控制措施包括测定变异系数、Bland Altman图和Pittman检验。使用双因素方差分析（ANOVA）和成对事后Scheffe检验分析结果。结果实验条件通过质量控制检查。双因素方差分析结果表明，无细胞上清液对生物膜形成的抑制作用比其细菌对应物更强且显著（双因素方差分析p=3.8×10（-6））。此外，含有乳酸杆菌的益生菌制剂（Accuflora®和Culturelle®）显著减少了生物膜形成，尤其是上清液形式。结论市售含乳酸杆菌的益生菌对C.白色念珠菌在假牙上形成生物膜。我们的研究结果的机制和临床意义需要通过更大的体内研究来解决。
PURPOSE There is a burgeoning recognition and interest in the potential of probiotics in the treatment and prevention of oral candidiasis associated with the use of dentures. Our aim was to investigate if commercially available over-the-counter probiotics can influence the ability of Candida albicans to form biofilms, which is considered a hallmark of the initiation and progression of oral candidiasis. METHODS We conducted a 2×5 factorial in vitro study to culture C. albicans on denture strips and challenge with one of the following four commercially available probiotics in bacterial or cell-free supernatant form: Accuflora®, Align®, Culturelle® and Sustenex®. C. albicans biofilm formation was studied in triplicates in all factorial combinations of the study and assessed qualitatively with fluorescence microscopy and quantitatively with tetrazolium salt (XTT) reduction assay. Quality control measures included determination of coefficient of variation, Bland Altman plots and Pittman's test. Results were analyzed using two-way analysis of variance (ANOVA) with pairwise post-hoc Scheffe's tests. RESULTS Our experimental conditions passed the quality control checks. Two-way ANOVA results indicated that cell-free supernatants provided a stronger and significant inhibitory effect on biofilm formation than their bacterial counterparts (2-way ANOVA p=3.8×10(-6)). Further, Lactobacillus-containing probiotic formulations (Accuflora® and Culturelle®) significantly reduced biofilm formation especially in supernatant form. CONCLUSION Commercially available probiotics that contain Lactobacilli species interfere with the in vitro ability of C. albicans to form biofilms on dentures. The mechanistic and clinical implications of our results need to be addressed by larger in vivo studies.