APPLICATION OF SPECIAL OLIGOTROPHIC MEDIA FOR CULTIVATION OF BACTERIAL COMMUNITIES ORIGINATED FROM ULTRAPURE WATER
APPLICATION OF SPECIAL OLIGOTROPHIC MEDIA FOR CULTIVATION OF BACTERIAL COMMUNITIES ORIGINATED FROM ULTRAPURE WATER
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DOI：
10.1556/amicr.60.2013.3.9
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发表时间：
2013-09-01
影响因子：
1.5
通讯作者：
Toth, Erika M.
中科院分区：
文献类型：
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作者：
Keki, Zsuzsa;Grebner, Kitti;Toth, Erika M.
由于常用介质的选择性，在实验室条件下培养超纯水中的细菌非常困难。在这项研究中，开发了 5 种新介质（合成和复合）来揭示源自匈牙利发电厂水净化系统的超纯水的细菌群落，该系统已使用传统介质进行了研究。新培养基的成分倾向于重现所研究水的营养缺乏条件，因此培养基是高度贫营养的。从 5 种不同的培养基中总共分离出 122 株细菌。根据ARDRA分组，选择27株菌株进行部分16S rRNA基因序列分析。结果表明，所应用的培养基强烈影响可培养细菌群落的组成。与之前应用的任何其他培养方法或培养基相比，可以从相同的超纯水系统中分离出更大规模的α-变形菌（中生根瘤菌属、不动杆菌属、甲基杆菌属）和许多放线菌属（莱夫索尼亚属、微杆菌属、分枝杆菌属）。此外，可以从所研究的水净化系统中分离出两种新的细菌类群。
Because of the selectivity of the commonly used media it is very difficult to cultivate bacteria inhabiting ultrapure waters under laboratory conditions. In this study 5 new media (synthetic and complex) were developed to reveal bacterial community of the ultrapure water originated from the water purification system of a Hungarian power plant which was studied already with using traditional media. Composition of the new media tends to reproduce the nutrient deficient conditions of the investigated water, therefore media were highly oligotrophic. Altogether 122 bacterial strains were isolated from the 5 different media. Based on ARDRA grouping 27 strains were chosen for the partial 16S rRNA gene sequence analysis. The results showed that the applied media strongly influence the composition of the cultivable bacterial community. A larger scale of alpha-Proteobacteria (Mesorhizobium spp., Ancylobacter sp., Methylobacterium sp.) and many Actinobacteria (Leifsonia sp., Microbacterium spp., Mycobacterium spp.) could be isolated from the same ultrapure water system than with any other cultivation methods or media applied before. Moreover, two novel bacterial taxa could be isolated from the studied water purification system.