Astragaloside IV alleviates mouse slow transit constipation by modulating gut microbiota profile and promoting butyric acid generation
Astragaloside IV alleviates mouse slow transit constipation by modulating gut microbiota profile and promoting butyric acid generation
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DOI：
10.1111/jcmm.15586
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发表时间：
2020-08-01
影响因子：
5.3
通讯作者：
Lin, Hongcheng
中科院分区：
文献类型：
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作者：
He, Qiulan;Han, Changpeng;Lin, Hongcheng
肠道微生物群和短链脂肪酸（SCFA）与各种人类疾病的发展有关。在这项研究中，我们研究了黄芪甲苷IV在调节小鼠肠道微生物群结构和SCFAs产生以及慢传输型便秘（STC）中的作用。用洛哌丁胺建立STC小鼠模型，观察黄芪甲苷的治疗作用。采用16 S rRNA基因测序和气相色谱-质谱联用技术分析了微生物群落结构和SCFA含量。丁酸盐对STC的影响使用小鼠模型和Cajal细胞（ICC）来评估。黄芪甲苷能促进STC小鼠排便，改善肠蠕动，抑制ICC丢失，减轻结肠病变。STC小鼠肠道微生物群结构的改变，如减少的罗伊氏乳杆菌多样性，在甘草皂苷IV治疗后得到改善。此外，黄芪甲苷上调丁酸和戊酸，但减少异戊酸，在STC小鼠粪便。丁酸盐通过调节AKT-NF-κ B信号通路促进排便，改善肠道运动，并增强ICC增殖。黄芪甲苷通过调节肠道菌群结构和产生丁酸，促进STC小鼠的肠道传输，抑制ICC丢失。
Gut microbiota and short-chain fatty acids (SCFAs) are associated with the development of various human diseases. In this study, we examined the role of astragaloside IV in modulating mouse gut microbiota structure and the generation of SCFAs, as well as in slow transit constipation (STC). An STC model was established by treating mice with loperamide, in which the therapeutic effects of astragaloside IV were evaluated. The microbiota community structure and SCFA content were analysed by 16S rRNA gene sequencing and gas chromatography-mass spectrometry, respectively. The influence of butyrate on STC was assessed using a mouse model and Cajal cells (ICC). Astragaloside IV promoted defecation, improved intestinal mobility, suppressed ICC loss and alleviated colonic lesions in STC mice. Alterations in gut microbiota community structure in STC mice, such as decreasedLactobacillus reuteridiversity, were improved following astragaloside IV treatment. Moreover, astragaloside IV up-regulated butyric acid and valeric acid, but decreased isovaleric acid, in STC mouse stools. Butyrate promoted defecation, improved intestinal mobility, and enhanced ICC proliferation by regulating the AKT-NF-kappa B signalling pathway. Astragaloside IV promoted intestinal transit in STC mice and inhibited ICC loss by regulating the gut microbiota community structure and generating butyric acid.