Development of a direct competitive enzyme-linked immunosorbent assay using a sensitive monoclonal antibody for bisphenol A.
Development of a direct competitive enzyme-linked immunosorbent assay using a sensitive monoclonal antibody for bisphenol A.
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DOI：
10.1089/hyb.2010.0079
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发表时间：
2011-02
期刊：
影响因子：
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通讯作者：
C. Ju;Youhua Xiong;A. Gao;Tang-bin Yang;Lei Wang
中科院分区：
文献类型：
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作者：
C. Ju;Youhua Xiong;A. Gao;Tang-bin Yang;Lei Wang
为了建立一种基于免疫分析的双酚A （BPA）检测方法，我们以BPA的羧基衍生物为免疫原制备了单克隆抗体（MAb）。以双酚a和丁二酸酐为原料，合成了双酚a - hs。用BPA-HS-BSA偶联物免疫小鼠。单抗是从杂交瘤中获得的。此外，我们发现单抗对BPA具有高度特异性。检测限在测定缓冲液中约为0.05 ng mL(-1) (ppb)，在水样中约为0.1 ng mL(-1) （ppb）。水样中BPA的回收率为90.8% ~ 114%，变异系数为15.6% ~ 39.4%。因此，ELISA法是一种快速、高通量的检测水产品中BPA的筛选工具。
To set up an immunoassay-based method to detect Bisphenol A (BPA), we generated a monoclonal antibody (MAb) using a specially designed carboxyl derivative of BPA as the immunogen. BPA-HS was synthesized by reaction using BPA and succinic anhydride. The mice were immunized with the BPA-HS-BSA conjugate. The MAb was obtained from a hybridoma. In addition, we showed that the MAb was highly specific for BPA. The limit of detection was approximately 0.05 ng mL(-1) (ppb) in assay buffer and 0.1 ng mL(-1) (ppb) in water samples. The recoveries of BPA for water samples were from 90.8% to 114%, and coefficients of variation were from 15.6% to 39.4%. Thus, the ELISA method is a rapid and high throughput screening tool to detect BPA in water products.