A HOUSEKEEPING GENE ON THE X-CHROMOSOME ENCODES A PROTEIN SIMILAR TO UBIQUITIN
A HOUSEKEEPING GENE ON THE X-CHROMOSOME ENCODES A PROTEIN SIMILAR TO UBIQUITIN
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DOI：
10.1073/pnas.85.3.851
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发表时间：
1988-02-01
影响因子：
11.1
通讯作者：
ALCALAY, M
中科院分区：
文献类型：
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作者：
TONIOLO, D;PERSICO, M;ALCALAY, M
在G6PD基因下游40千碱基处的X染色体基因Xq28被分离并测序。这个基因，我们命名为GdX，横跨大约3.5千碱基的基因组DNA。GdX是一个单拷贝基因，在进化中是保守的，具有“看家”基因的特征。在它的5'末端，一簇CpG二核苷酸在不活跃的X染色体上甲基化，在活跃的X染色体上未甲基化。GdX基因可以编码一种含有157个氨基酸的蛋白质。GdX的残基1-74与泛素具有43%的同源性，泛素是一种高度保守的76个氨基酸的蛋白。GdX的cooh末端部分在其中心部分（残基110-128）与甲状腺球蛋白的cooh末端激素原位点同源。GdX蛋白的结构组织表明，除了泛素基因外，还存在一个基因家族，可能通过蛋白质-蛋白质识别在关键的细胞过程中发挥特定作用。
An X chromosome gene located 40 kilobases downstream from the G6PD gene, at Xq28, was isolated and sequenced. This gene, which we named GdX, spans about 3.5 kilobases of genomic DNA. GdX is a single-copy gene, is conserved in evolution, and has the features of a "house-keeping" gene. At its 5'' end, a cluster of CpG dinucleotides is methylated on the inactive X chromosome and unmethylated on the active X chromosome. The GdX gene can code for a 157 amino acid protein, GdX. Residues 1-74 of GdX show 43% identity to ubiquitin, a highly conserved 76 amino acid protein. The COOH-terminal moiety of GdX is characterized in its central part (residues 110-128) by a sequence homologous to the COOH-terminal hormonogenic site of thyroglobulin. The structural organization of the GdX protein suggests the existence of a family of genes, in addition to the ubiquitin gene, that could play specific roles in key cellular processes, possibly through protein-protein recognition.