Gene transcription analysis in lesional skin of canine epitheliotropic cutaneous lymphoma using quantitative real-time RT-PCR
Gene transcription analysis in lesional skin of canine epitheliotropic cutaneous lymphoma using quantitative real-time RT-PCR
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DOI：
10.1016/j.vetimm.2011.08.012
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发表时间：
2011-12-15
影响因子：
1.8
通讯作者：
Maeda, Sadatoshi
中科院分区：
文献类型：
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作者：
Chimura, Naoki;Kondo, Naho;Maeda, Sadatoshi
犬嗜上皮性皮肤淋巴瘤(CECL)是一种以皮肤中具有特殊嗜性的肿瘤淋巴细胞为特征的疾病。趋化因子和趋化因子受体之间的相互作用严格控制着淋巴细胞的迁移，这可能参与了CECL的发病机制。本研究采用实时定量RT-PCR方法检测了几种趋化因子(CCL17、CCL19、CCL21、CCL22、CCL27、CCL28和CXCL10)和趋化因子受体(CCR4、CCR7、CCR10和CXCR3)在cECL皮损中的转录水平。为了检测积聚的肿瘤淋巴细胞亚群，我们还检测了1型(干扰素-γ、IL-12p35、IL-12p40和LT-α)和2型(IL-4和IL-13)细胞因子和细胞毒标志物(穿孔素和颗粒酶B)的转录水平。结果发现，与正常皮肤相比，皮损组织中CCL19、CXCL10、CCR4、CCR7、CCR10和CXCR3基因转录水平升高，CCL27基因转录水平降低(P<0.05)。
Canine epitheliotropic cutaneous lymphoma (cECL) is characterized by infiltration of neoplastic lymphocytes in the skin with a specific tropism for the epidermis. Migration of lymphocytes is strictly controlled by interactions between chemokines and chemokine receptors, which may be involved in the pathogenesis of cECL. In this study, we investigated mRNA transcription levels of several chemokines (CCL17, CCL19, CCL21, CCL22, CCL27, CCL28 and CXCL10) and chemokine receptors (CCR4, CCR7, CCR10 and CXCR3) in lesional skin of cECL by quantitative real-time RT-PCR. To examine the subsets of accumulating neoplastic lymphocytes, we also investigated transcription levels of type-1 (IFN-gamma, IL-12p35, IL-12p40 and LT-alpha) and type-2 (IL-4 and IL-13) cytokines and cytotoxic markers (perforin and granzyme B). We found that the lesional skin had higher mRNA transcription of CCL19, CXCL10, CCR4, CCR7, CCR10 and CXCR3 and lower transcription of CCL27 than healthy dog skin (p