DETAILED METHODOLOGY AND IMPLEMENTATION OF A SEMIAUTOMATED SERIAL DILUTION MICROTECHNIQUE FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING
DETAILED METHODOLOGY AND IMPLEMENTATION OF A SEMIAUTOMATED SERIAL DILUTION MICROTECHNIQUE FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING
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DOI：
10.1128/aem.20.1.46-53.1970
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发表时间：
1970-01-01
期刊：
影响因子：
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通讯作者：
SELEPAK, ST
中科院分区：
文献类型：
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作者：
MACLOWRY, JD;JAQUA, MJ;SELEPAK, ST
描述了用于进行连续稀释抗菌敏感性研究的半自动微技术的详细方法和实施。对所有重要的临床分离株常规进行一系列抗菌药物的定量敏感性研究。结果以每毫升肉汤微克为单位报告。提出了将抗菌药物的标准剂量与预期血液和尿液浓度相关的指南，以方便定量数据的使用。这种微技术用于测量血清和其他体液中抗菌剂的水平，以记录特定疗程所达到的水平。该技术具有高度可重复性，与标准玻璃管技术具有高度相关性，并且速度至少比标准玻璃管技术快 10 倍。
The detailed methodology and implementation of a semiautomatic microtechnique for performing serial dilution antimicrobial susceptibility studies are described. Quantitative susceptibility studies to a battery of antimicrobials are performed routinely on all significant clinical isolates. Results are reported as the minimal inhibitory concentration in micrograms per milliliter of broth. Guidelines relating standard doses of antimicrobials with expected blood and urine levels are presented to facilitate the use of the quantitative data. This microtechnique is used to measure serum and other body fluid levels of antimicrobial agents to document the level attained with a specific course of therapy. This technique is highly reproducible and has a high correlation with, and is at least 10 times faster than, standard glass tube techniques.