Mouse major histocompatibility complex (H-2) and fetal lung development: implications for human pulmonary maturation.
Mouse major histocompatibility complex (H-2) and fetal lung development: implications for human pulmonary maturation.
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小鼠主要组织相容性复合体 (H-2) 和胎儿肺发育：对人类肺成熟的影响。
DOI：
10.1002/ajmg.1320350124
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Melnick,M
中科院分区：
文献类型：
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作者：
Hu,CC;Jaskoll,TF;Minkin,C;Melnick,M
使用C57/10 Sn（B10，H-2b）和B10.A/SgSn（B10.A，H-2a）同源小鼠，我们测量了1）小鼠发育期间内源性肺皮质酮的水平;2）妊娠第17天肺形态成熟的程度，有或没有皮质类固醇治疗; 3）母体对正常肺发育和胎儿对皮质类固醇反应的影响。我们的研究结果表明，胎儿B10（H-2b）和B10.A（H-2a）小鼠的内源性激素水平随着时间的推移逐渐增加;在整个妊娠中期至晚期，两种品系小鼠肺中激素的可检测量几乎相同。通过形态测定法评价肺成熟程度，发现B10.A小鼠肺比B10小鼠肺更不成熟。在妊娠第12天皮质类固醇治疗后，H-2 alungs等于或比H-2 blungs更成熟。我们还比较了来自正反交的杂合小鼠肺（B10. B10.A，B/a和B10.A.B10，a/B）。具有母体来源的H-2a单倍型的小鼠比具有母体来源的H-2b单倍型的小鼠具有更少的成熟肺，这表明母体效应。当给予外源性激素时，所有杂合子小鼠肺的成熟度增加，而不管H-2a单倍型的起源如何。经处理的a/B或B/a肺比纯合子B/B肺更成熟，比纯合子a/a肺更不成熟。我们的结论是，进行性肺成熟与H-2复合物或其附近的基因相关，对皮质类固醇的反应能力也是如此。
Using C57/10Sn (B10, H‐2b) and B10.A/SgSn (B10.A, H‐2a) congenic mice, we measured 1) the level of endogenous pulmonary cor‐ticosterone during mouse development;2) the degree of lung morphological maturation on gestation day 17, with or without corticosteroid treatment; and 3) the maternal influence on normal lung development and fetal response to corticosteroids. The results of our study indicate that there was a progressive increase in the level of endogenous hormone with time in fetal B10 (H‐2b) and B10.A (H‐2a) mice; throughout mid‐ to late gestation, the detectable amount of hormone was almost identical in lungs of both strains. Evaluating the degree of lung maturation by morphometry, B10.A mouse lungs were found to be less mature than B10 mouse lungs. Following corticosteroid treatment on day 12 of gestation, H‐2alungs were equal to or more mature than H‐2blungs. We also compared heterozygous mouse lungs from reciprocal crosses (B10. B10.A, b/a and B10.A.B10, a/b). Mice with a maternally derived H‐2ahaplotype had less mature lungs than those with a maternally derived H‐2bhaplotype, suggesting a maternal effect. When exogenous hormone was administered, all heterozygous mouse lungs increased in maturity regardless of the origin of the H‐2ahaplotype. The treated a/b or b/a lungs were more mature than homozygous b/b and less mature than homozygous a/a lungs. We conclude that progressive lung maturation is associated with a gene (s) at or near the H‐2 complex, as is the ability to respond to corticosteroids.