Immunohistochemistry for Matrix Proteins in newly formed Mandibular Condylar Cartilage in a Human Fetus
Immunohistochemistry for Matrix Proteins in newly formed Mandibular Condylar Cartilage in a Human Fetus
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人类胎儿新形成的下颌髁软骨基质蛋白的免疫组织化学分析
DOI：
10.4172/2161-0940.1000262
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
G. Murakami
中科院分区：
文献类型：
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作者：
S. Shibata;Zin Zw;Y. Jin;P. Zhao;G. Murakami
对孕9周新生的下颌髁突软骨（冠臀长37 mm）进行了化学分析。新形成的软骨中的细胞和软骨后部的浓缩的间充质细胞显示碱性磷酸酶免疫反应性，证实人类的下颌髁突软骨也来源于骨膜样组织，如在啮齿动物中所证明的。新形成的髁突软骨同时表达I型和II型胶原蛋白、聚集蛋白聚糖、多能蛋白聚糖和生腱蛋白-C以及透明质酸染色的免疫反应性，但不表达X型胶原蛋白。因此，新形成的髁突软骨具有纤维软骨的特征，但在啮齿动物次级软骨中识别的快速分化为肥大软骨细胞的特征在人类中不明显。
A newly formed mandibular condylar cartilage at 9 weeks of gestation (37mm crown rump length) was analyzed immunohistochemically. The cells in newly formed cartilage and condensed mesenchymal cells posterior to the cartilage showed alkaline phosphatase immunoreactivity, confirming the mandibular condylar cartilage in human is also derived from periosteum-like tissue, as demonstrated in rodents. The newly formed condylar cartilage simultaneously expresses immunoreactivity for collagen types I and II, aggrecan, versican, and tenascin-C as well as hyaluronan staining but not for collagen type X. Thus newly-formed condylar cartilage has fibrocartilaginous characteristics, but the characteristic that rapidly differentiated into hypertrophic chondrocytes, which is recognized in rodent secondary cartilage, is not conspicuous in human.
发育中的小鼠肢芽软骨中多功能蛋白聚糖、聚集蛋白聚糖和连接蛋白的原位杂交和免疫组织化学，以及透明质酸的组织化学。
DOI：
--
发表时间：
2003
期刊：
J Anat. 203(4)
影响因子：
--
作者：
Shibata S;Fukada K;Imai H;Abe T;Yamashita Y.
通讯作者：
Yamashita Y.