Preparation of Viable islet Cells from Dogs by a New Method
Preparation of Viable islet Cells from Dogs by a New Method
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用新方法制备狗活胰岛细胞
DOI：
10.2337/diab.30.5.455
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发表时间：
1981
期刊：
影响因子：
7.7
通讯作者：
R. Merrell
中科院分区：
文献类型：
--
作者：
A. Horaguchi;R. Merrell
本文介绍了一种从正常犬胰腺中分离胰岛细胞的新方法，即采用胶原酶灌注胰管的方法。将腺泡组织暴露于最高浓度的胶原酶导致胰岛产量提高，腺泡污染减少。在通过导管灌注选择性消化腺体后，机械解离释放单个细胞和团块。通过胰岛素产率和淀粉酶消耗对该过程进行分析，表明B细胞回收率为57%，B细胞浓度富集6倍。这些细胞已经作为自体移植物移植到胰腺切除术后的狗身上。在7只移植犬中，5只术后恢复正常。
A new method for the preparation of viable islet cells from the normal dog pancreas is described, based on the perfusion of the pancreatic duct with collagenase. Exposure of the acinar tissue to the highest concentrations of collagenase results in improved islet yield with decreased acinar contamination. After the selective digestion of the gland by ductal perfusion, mechanical dissociation liberates single cells and clumps. Analysis of the procedure by insulin yield and amylase attrition indicates a 57% B-cell recovery and a sixfold enrichment in B-cell concentration. The cells have been transplanted as autografts into dogs after pancreatectomy. In 5 of 7 transplanted dogs, normoglycemia was achieved postoperatively.