Analysis of Serum miRNAs in Alzheimer's Disease
Analysis of Serum miRNAs in Alzheimer's Disease
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阿尔茨海默病血清 miRNA 分析
DOI：
10.1177/15333175211021712
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发表时间：
2021-06-02
影响因子：
3.4
通讯作者：
Ma, Tao
中科院分区：
文献类型：
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作者：
Lu, Liu;Dai, Wen-Zhuo;Ma, Tao
本文旨在分析阿尔茨海默病（Alzheimer disease，AD）中的microRNA（microRNA，miRNA）特征，寻找其显著表达的miRNAs及其靶基因，对已确认的基因进行功能富集分析，并探讨其潜在的药物治疗作用。从Gene Expression Omnibus数据库下载基因表达谱数据的miRNA表达信息。总数据样本量为1309个，其中AD样本1021个，正常样本288个。共获得21个差异表达的miRNAs，其中16个差异表达的miRNAs为16个，（hsa-miR-6761 - 3p、hsa-miR-6747 - 3p、hsa-miR-6875 - 3p、hsa-miR-6754 - 3p、hsa-miR-6736 - 3p、hsa-miR-6762 - 3p、hsa-miR-6787 - 3p、hsa-miR-208a-5p、hsa-miR-6740 - 3p、hsa-miR-6778 - 3p、hsa-miR-595，hsa-miR-6753 - 3p、hsa-miR-4747 - 3p、hsa-miR-3646、hsa-miR-6716 - 3p和hsa-miR-4435）在AD中上调，5个（hsa-miR-125a-3p、hsa-miR-22 - 3p、hsa-miR-24 - 3p、hsa-miR-6131和hsa-miR-125b-1 - 3p）在AD中下调。共预测了6个miRNA（hsa-miR-595、hsa-miR-3646、hsa-miR-4435、hsa-miR-125 a-3p、hsa-miR-22 - 3p和hsa-miR-24 - 3p）和78个miRNA疾病相关基因子网络，获得了116个ceRNA调控关系对和ceRNA调控网络。富集分析结果提示，AD中差异表达的几种miRNAs主要作用靶通路是丝裂原活化蛋白激酶信号通路。根据药物-基因相互作用数据库2.0的预测结果，我们得到了53对药物-基因相互作用，包括7个基因（PTGS 2、EGFR、CALM1、PDE 4D、FGFR 2、HMGCR、cdk 6）和53种药物。我们希望我们的研究结果有助于找到一种可行的方法来预防，延迟发病，诊断和治疗AD。
This paper was aimed to analyze the microRNA (miRNA) signatures in Alzheimer disease (AD) and find the significant expressions of miRNAs, their target genes, the functional enrichment analysis of the confirmed genes, and potential drug treatment. The miRNA expression information of the gene expression profile data was downloaded from the Gene Expression Omnibus database. The total data sample size is 1309, including 1021 AD samples and 288 normal samples. A total of 21 differentially expressed miRNAs were obtained, of which 16 (hsa-miR-6761-3p, hsa-miR-6747-3p, hsa-miR-6875-3p, hsa-miR-6754-3p, hsa-miR-6736-3p, hsa-miR-6762-3p, hsa-miR-6787-3p, hsa-miR-208a-5p, hsa-miR-6740-3p, hsa-miR-6778-3p, hsa-miR-595, hsa-miR-6753-3p, hsa-miR-4747-3p, hsa-miR-3646, hsa-miR-6716-3p and hsa-miR-4435) were up-regulated and 5 (hsa-miR-125a-3p, hsa-miR-22-3p, hsa-miR-24-3p, hsa-miR-6131 and hsa-miR-125b-1-3p) were down-regulated in AD. A total of 6 miRNAs (hsa-miR-595, hsa-miR-3646, hsa-miR-4435 hsa-miR-125a-3p, hsa-miR-22-3p and hsa-miR-24-3p) and 78 miRNA-disease-related gene sub-networks were predicted, and 116 ceRNA regulatory relationship pairs, and the ceRNA regulatory network were obtained. The results of enrichment analysis suggested that the main target pathways of several miRNAs differentially expressed in AD were mitogen-activated protein kinase signal pathway. According to the prediction results of Drug-Gene Interaction database 2.0, we obtained 53 pairs of drug-gene interaction, including 7 genes (PTGS2, EGFR, CALM1, PDE4D, FGFR2, HMGCR, cdk6) and 53 drugs. We hope our results are helpful to find a viable way to prevent, delay the onset, diagnose, and treat AD.