RAPID FOCUS REDUCTION NEUTRALIZATION TEST OF INFLUENZA-A AND INFLUENZA-B VIRUSES IN MICROTITER SYSTEM
RAPID FOCUS REDUCTION NEUTRALIZATION TEST OF INFLUENZA-A AND INFLUENZA-B VIRUSES IN MICROTITER SYSTEM
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DOI：
10.1128/jcm.28.6.1308-1313.1990
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发表时间：
1990-06-01
影响因子：
9.4
通讯作者：
UEDA, S
中科院分区：
文献类型：
--
作者：
OKUNO, Y;TANAKA, K;UEDA, S
开发了甲型和B型流感病毒的快速中和试验。在该方法中，使用96孔组织培养板制备细胞单层，并使用过氧化物酶-抗过氧化物酶染色技术观察感染这些病毒的病灶。在胰蛋白酶和黄蓍胶的存在下，感染后1天出现清晰的病灶。观察到病毒稀释度与病灶数量之间存在线性关系。当测定某些试验血清中的中和抗体时，观察到病灶法获得的滴度与普通空斑法获得的滴度之间具有良好的相关性。此外，许多血清标本进行了调查的中和试验，它被证明是有用的流感血清学研究。
A rapid neutralization test for influenza A and B viruses was developed. In this method, a 96-well tissue culture plate was used for the preparation of cell monolayers and in the peroxidase-antiperoxidase staining technique was used for the visualization of foci infected with these viruses. In the presence of trypsin and tragacanth gum, clear foci developed 1 day after infection. A linear relationship between virus dilutions and numbers of foci was observed. When neutralizing antibodies in some test sera were assayed, a good correlation was observed between the titers obtained by the focus method and those obtained by the ordinary plaque method. In addition, many serum specimens were investigated by the neutralization test, and it was demonstrated that the test is useful for serological studies of influenza.