Claudin expression in gastric adenocarcinomas: a tissue microarray study with prognostic correlation
Claudin expression in gastric adenocarcinomas: a tissue microarray study with prognostic correlation
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DOI：
10.1016/j.humpath.2005.05.019
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发表时间：
2005-08-01
期刊：
影响因子：
3.3
通讯作者：
Moss, SF
中科院分区：
文献类型：
--
作者：
Resnick, MB;Gavilanez, M;Moss, SF
claudins包含一个多基因家族的膜蛋白，在紧密连接的形成中发挥重要作用。在一些恶性肿瘤中已经描述了某些密蛋白表达的异常。我们的目的是确定一个大系列的美国胃癌患者中claudins 1，3和4以及ZO-1的表达模式，并将表达与临床病理和预后变量相关联。组织微阵列是从146例远端胃腺癌患者（61例肠腺癌和85例弥漫或混合亚型）的石蜡标本中制备的。此外，大多数病例均取正常粘膜和肠上皮化生组织。对微阵列进行紧密连接蛋白1、3和4以及ZO-1染色，并使用3点量表确定染色强度。中度claudin 1和ZO-1膜染色，而只有局灶性弱claudin 3和4膜染色存在于正常胃上皮。claudins 1、3、4和ZO-1在肠腺癌中的表达率分别为74%、48%、62%和74%，而在弥漫型腺癌中的表达率分别为46%、24%、45%和36%。
The claudins comprise a multigene family of integral membrane proteins, which play a major role in tight junction formation. Aberrations in the expression of certain claudins have been described in a number of malignancies. Our aims were to determine the expression pattern of claudins 1, 3, and 4 as well as ZO-1 in a large series of US patients with gastric cancer and to correlate expression with clinicopathologic and prognostic variables. Tissue microarrays were created from paraffinized samples from 146 patients with distal gastric adenocarcinomas (61 intestinal and 85 diffuse or mixed subtypes). In addition, cores of normal mucosa and intestinal metaplasia were taken from most cases. The microarrays were stained for claudins 1, 3, and 4 and ZO-1, and the intensity of staining was determined using a 3-point scale. Moderate claudin 1 and ZO-1 membranous staining were present, whereas only focal weak claudin 3 and 4 membranous staining was present in normal gastric epithelium. Moderate to strong staining of claudins 1, 3, 4, and ZO-1 was detected in 74%, 48%, 62%, and 74% of the intestinal but in only 46%, 24%, 45%, and 36% of the diffuse subtype of adenocarcinomas (P