Lipid-protein interactions with the Na,K-ATPase
Lipid-protein interactions with the Na,K-ATPase
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DOI：
10.1016/j.chemphyslip.2006.02.018
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发表时间：
2006-06-01
影响因子：
3.4
通讯作者：
Marsh, Derek
中科院分区：
文献类型：
--
作者：
Esmann, Mikael;Marsh, Derek
综述了利用电子自旋共振光谱法结合自旋标记脂质对脂质与膜 Na,K-ATP 酶相互作用的研究。除了有关蛋白质相关脂质链的构型和旋转动力学的信息之外，还可以确定脂质-蛋白质界面处的脂质化学计量、选择性和交换动力学。这些参数，特别是化学计量和选择性，与 Na,K-ATP 酶的膜内结构直接相关，可用于检查广泛胰蛋白酶处理的制剂的完整性。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
Studies of lipid interactions with membranous Na,K-ATPase by using electron spin resonance spectroscopy in conjunction with spin-labelled lipids are reviewed. The lipid stoichiometry, selectivity and exchange dynamics at the lipid-protein interface can be determined, in addition to information on the configuration and rotational dynamics of the protein-associated lipid chains. These parameters, particularly the stoichiometry and selectivity, are related directly to the intramembranous structure of the Na,K-ATPase, and can be used to check the integrity of extensively trypsinised preparations. (c) 2006 Elsevier Ireland Ltd. All rights reserved.