Pure docosahexaenoic acid can improve depression behaviors and affect HPA axis in mice.
Pure docosahexaenoic acid can improve depression behaviors and affect HPA axis in mice.
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DOI：
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发表时间：
2012-11
影响因子：
3.3
通讯作者：
L. Jiang;Q-Y Liang;Y. Shi
中科院分区：
文献类型：
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作者：
L. Jiang;Q-Y Liang;Y. Shi
目的通过下丘脑-垂体-肾上腺轴（HPA轴）研究二十二碳六烯酸（DHA）是否具有重要的抗抑郁作用。方法小鼠随机分为对照组和DHA组。DHA饮食组每天给予DHA饮食，连续50天。进行强迫游泳试验和悬尾试验。测定下丘脑和红细胞脂肪酸及下丘脑单胺类神经递质含量;测定血清、下丘脑和垂体皮质酮、促肾上腺皮质激素和促肾上腺皮质激素释放因子含量。结果（1）在强迫游泳实验中，DHA显著缩短小鼠不动时间，增加小鼠游泳时间和爬高时间。在悬尾试验中，DHA显著缩短小鼠不动时间。(2)DHA可提高n-3/n-6比值结果表明：（1）添加DHA组大鼠下丘脑和红细胞脂肪酸中多不饱和脂肪酸（PUFA）和DHA含量显著降低，（2）添加DHA组大鼠下丘脑和红细胞脂肪酸中5-羟色胺、5-羟吲哚乙酸和多巴胺含量显著升高，（3）添加DHA组大鼠血清皮质酮和促肾上腺皮质激素水平显著降低，分别降低20.23%和25.13%;显著提高血清促肾上腺皮质激素释放因子水平21.92%。（5）日粮中添加DHA使下丘脑和垂体组织中的促肾上腺皮质激素释放因子水平分别降低了30.83%和29.75%。在下丘脑中，DHA显著降低促肾上腺皮质激素水平，降低幅度为19.14%，但对垂体促肾上腺皮质激素水平的影响不显著。结论DHA具有抗抑郁作用。此外，DHA对抑郁症有多种影响，包括单胺神经递质系统，红细胞膜和HPA轴。
OBJECTIVE Aim of this study was to identify whether docosahexaenoic acid (DHA) has a vital antidepressant by hypothalamic-pituitary-adrenal axis (HPA axis) or not. METHODS Mice were divided into 2 groups: control group and DHA dietary group. DHA dietary group was treated with DHA dietary everyday for consecutive 50 days. The forced swimming test and tail suspension test were conducted. Hypothalamic and erythrocyte fatty acids and monoamine neurotransmitters levels in hypothalamus were assayed; corticosterone, adrenocorticotropic hormone and corticotropin-releasing factor in serum, hypothalamus and pituitary were assayed, respectively. RESULTS (1) In the forced swimming test, DHA dietary significantly decreased immobility time, whereas swimming time and climbing time were increased. In tail suspension test, DHA dietary significantly shortened immobility time. (2) DHA dietary increased the ration of n-3/n-6 (polyunsaturated fatty acids) and DHA level in hypothalamic and erythrocyte fatty acids; (3) DHA dietary significantly increased 5-hydroxytryptamine, 5-hydroxyindoleacetic acid and dopamine levels in hypothalamus; (4) DHA dietary significantly decreased serum corticosterone level by 20.23% and serum adrenocorticotropic hormone level by 25.13%; significantly increased serum corticotropin releasing factor level by 21.92%. Besides, DHA dietary decreased arginine vasopressin level by 20.11% in hypothalamus, by 23.76% in pituitary tissues, respectively; (5) DHA dietary decreased corticotropin-releasing factor levels by 30.83% in hypothalamus, by 29.75% in pituitary tissues, respectively. In hypothalamus, DHA dietary decreased significantly adrenocorticotropic hormone level by 19.14%, but insignificantly decreased adrenocorticotropic hormone level in pituitary. CONCLUSIONS DHA shows an antidepressant property. Moreover, DHA has multiple effects on depression including the monoamine neurotransmitter systems, red blood cell membranes and HPA axis.