Eukaryotic mutagenesis and translesion replication dependent on DNA polymerase ζ and Rev 1 protein
Eukaryotic mutagenesis and translesion replication dependent on DNA polymerase ζ and Rev 1 protein
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DOI：
10.1042/bst0290187
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发表时间：
2001-05-01
影响因子：
3.9
通讯作者：
Maher, VM
中科院分区：
文献类型：
--
作者：
Lawrence, CW;Maher, VM
转录复制是一种机制，其采用专门的DNA聚合酶来促进在被未修复的DNA损伤的存在所阻断的叉处的持续新生链延伸。至少在酿酒酵母中，这一过程对细胞耐受DNA损伤的能力贡献不大，但却是DNA损伤诱导的取代和移码以及自发突变的主要来源。酵母中许多类型的DNA损伤后的转录复制依赖于DNA聚合酶zeta（pol zeta）和Rev1p的活性。Pol zeta存在于大多数但不是所有的真核生物中，而Rev1p似乎是普遍存在的。编码这些酶的基因在人类中被发现，并且似乎执行与酵母中类似的功能。
Translesion replication is a mechanism that employs specialized DNA polymerases for promoting continued nascent strand extension at forks blocked by the presence of unrepaired DNA damage. In Saccharomyces cerevisiae at least, this process contributes only modestly to the ability of cells to tolerate DNA damage, but is a major source of DNA-damage-induced substitutions and frameshifts, and of spontaneous mutations. Translesion replication past many types of DNA damage in yeast depends on the activities of DNA polymerase zeta (pol zeta) and Rev1p. Pol zeta is found in most, but not all, eukaryotes investigated, whereas Rev1p appears to be universal. Genes encoding these enzymes are found in humans, and appear to perform functions similar to those in yeast.