Pollination-induced ethylene in carnation - Role of stylar ethylene in corolla senescence
Pollination-induced ethylene in carnation - Role of stylar ethylene in corolla senescence
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DOI：
10.1104/pp.115.1.205
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发表时间：
1997-09-01
期刊：
影响因子：
7.4
通讯作者：
Woodson, WR
中科院分区：
文献类型：
--
作者：
Jones, ML;Woodson, WR
康乃馨（Dianthus caryophyllus L.）cv白色Sim）花粉与雌蕊之间的细胞间通讯诱导子房发育和花冠衰老。由花柱产生的升高的乙烯是第一个可测量的授粉后反应。随后是来自其他花器官的乙烯生产浪潮。为了研究授粉花中乙烯生物合成的调节，我们测量了授粉后单个花器官中乙烯产生和1-氨基环丙烷-1-羧酸合酶和1-氨基环丙烷-1-羧酸氧化酶转录本的表达。授粉花柱的乙烯产量在时间上可以由三个不同的峰值来定义。通过从多花柱雌蕊群授粉单个花柱，确定未授粉花柱产生乙烯，其对应于从授粉花柱观察到的第一和第三峰。重氮环戊二烯处理抑制授粉花柱中的乙烯作用，防止授粉诱导的花冠衰老和乙烯产生的子房和花瓣。重氮环戊二烯处理降低了花柱乙烯产生的第二个高峰，完全抑制了第三个高峰的乙烯在授粉和未授粉的风格。花柱中的乙烯可能是授粉诱导的花冠衰老和子房发育的原因。
In carnation (Dianthus caryophyllus L. cv White Sim) cell to cell communication between the pollen and pistil induces ovary development and corolla senescence. The production of elevated ethylene by the style is the first measurable postpollination response. This is followed by a wave of ethylene production from the other floral organs. To investigate the regulation of ethylene biosynthesis in pollinated flowers we measured ethylene production and the expression of 1-aminocyclopropane-1-carboxylate synthase and 1-aminocyclopropane-1-carboxylate oxidase transcripts in individual floral organs after pollination. Ethylene production by pollinated styles can be defined temporally by three distinct peaks. By pollinating a single style from a multistyle gynoecium, it was determined that the unpollinated style produces ethylene that corresponds to the first and third peaks observed from a pollinated style. Inhibition of ethylene action in the pollinated style by diazocyclopentadiene treatment prevented both pollination-induced corolla senescence and ethylene production from the ovaries and petals. Treatment with diazocyclopentadiene decreased stylar ethylene production during the second peak and completely inhibited the third peak of ethylene in both pollinated and unpollinated styles. This later autocatalytic ethylene in styles is likely responsible for pollination-induced corolla senescence and ovary development.