LC-MS-based chemotaxonomic classification of wild-type Lespedeza sp and its correlation with genotype
LC-MS-based chemotaxonomic classification of wild-type Lespedeza sp and its correlation with genotype
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DOI：
10.1007/s00299-012-1319-8
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发表时间：
2012-11-01
影响因子：
6.2
通讯作者：
Lee, Choong Hwan
中科院分区：
文献类型：
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作者：
Kim, Young Mi;Lee, Joongku;Lee, Choong Hwan
本研究对胡枝子属（Lespedeza cyrtobotrya、L. bicolor、L. maximowiczii和Lespedeza cuneata） （Leguminosae） 39个标本进行了表型和基因典型分类。我们基于nrDNA （ITS）和cpDNA （trnL-trnF）组合序列构建了系统发育树，目的是对基因型进行分类。样本主要分为三种基因型。双色l和细胞质l混合在一个枝上，大叶l和楔形l分别聚集在种内。我们进行了液相色谱-电喷雾电离-质谱为基础的代谢物分析，以分类表型。聚类模式分析采用主成分分析（PCA），种间距离分析采用层次聚类分析（HCA）。根据PCA和HCA分析结果，叶片按物种分为4种表型。在遗传和化学分类方法上，两种间的距离最接近，而与其他3种的距离最远。此外，采用正交偏最小二乘判别分析，发现不同种间植物化学成分存在显著差异。通过鉴定不同的植物化学成分，我们对胞草和双色草进行了分类。有趣的是，叶和茎在化学分类上表现出不同的表型分类。其他三种植物的茎样不分物种均为混合，而胞浆草的茎样均为种内聚集。叶片的表型分类与基因型分类的一致性高于茎的表型分类。利用nrDNA （ITS）和cpDNA （trnL-trnF）组合序列对4种野生型胡枝子（lepedeza sp.）进行分类。采用液相色谱-质谱联用分析方法对胡枝子叶和茎进行了分类。
In this study, 39 specimens belonging to Lespedeza species (Lespedeza cyrtobotrya, L. bicolor, L. maximowiczii, and Lespedeza cuneata) (Leguminosae) were classified phenotypically and genotypically. We constructed a phylogenetic tree based on the combined nrDNA (internal transcribed spacer; ITS) and cpDNA (trnL-trnF) sequences with the aim of classifying the genotypes. Samples were mainly divided into three genotypes. Samples of L. cyrtobotrya and L. bicolor were mixed in a single branch, whereas samples of L. maximowiczii and L. cuneata were clustered within species, respectively. We performed a liquid chromatography-electrospray ionization-mass spectrometry-based metabolite profiling analysis to classify the phenotypes. Multivariate statistical analyses such as principal component analysis (PCA) and hierarchical clustering analysis (HCA) were used for the clustering pattern analysis and distance analysis between species, respectively. According to the PCA and HCA results, leaves were classified into four phenotypes according to species. In both the genetic and chemotaxonomic classification methods, the distance between L. cyrtobotrya and L. bicolor was the closest between species, and L. cuneata was the farthest away from the other three species. Additionally, orthogonal partial least squares-discriminant analysis was employed to identify significantly different phytochemicals between species. We classified L. cyrtobotrya and L. bicolor by identifying significantly different phytochemicals. Interestingly, leaves and stems showed different phenotypic classifications based on the chemotaxonomic classification. Stem samples of the other three species were mixed regardless of species, whereas L. cyrtobotrya stem samples were clustered within species. The phenotypic classification of leaves coincided more with the genotypic classification than that of stems.Key message We classified four wild-type Lespedeza sp. by analyzing the combined nrDNA (ITS) and cpDNA (trnL-trnF) sequences. We also classified leaves and stems of Lespedeza sp. by applying liquid chromatography-mass spectroscopy-based metabolite profiling.