Sequestrin, a CD36 recognition protein on Plasmodium falciparum malaria-infected erythrocytes identified by anti-idiotype antibodies.
Sequestrin, a CD36 recognition protein on Plasmodium falciparum malaria-infected erythrocytes identified by anti-idiotype antibodies.
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Sequestrin，一种由抗独特型抗体识别的恶性疟原虫疟疾感染红细胞上的 CD36 识别蛋白。
DOI：
10.1073/pnas.88.8.3175
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发表时间：
1991
影响因子：
11.1
通讯作者：
Jamieson,GA
中科院分区：
文献类型：
--
作者：
Ockenhouse,CF;Klotz,FW;Tandon,NN;Jamieson,GA
人内皮细胞表达的CD36分子是感染人恶性疟原虫的红细胞粘附的受体。CD36特异性单克隆抗体OKM 8可抑制疟疾感染的红细胞（IRBC）与纯化的CD36和表达CD36的细胞的粘附。单特异性多克隆抗独特型（抗Id）抗体，提出了对单克隆抗体OKM 8，表达的决定簇分子模拟CD36结合域的粘附IRBC。纯化的兔抗Id抗体与IRBC表面的免疫荧光反应，直接支持的野生型恶性疟原虫疟疾分离株的粘附，并抑制IRBC细胞粘附黑色素瘤细胞。一个约270 kDa的蛋白质免疫沉淀的抗Id抗体从表面标记和代谢标记的IRBC和竞争性抑制可溶性CD36。这些结果支持CD36是一种受体的假设，约270 kDa的蛋白，螯合蛋白，是一种互补的配体参与IRBC的粘附宿主细胞内皮。螯合蛋白是一种候选疟疾疫苗抗原，识别该分子的抗Id抗体可能用于脑型和重症恶性疟原虫疟疾的被动免疫治疗。
The CD36 molecule expressed by human endothelial cells is a receptor for the adhesion of erythrocytes infected with the human malaria parasite Plasmodium falciparum. A CD36-specific monoclonal antibody, OKM8, inhibits the adhesion of malaria-infected erythrocytes (IRBC) to purified CD36 and cells expressing CD36. Monospecific polyclonal anti-idiotype (anti-Id) antibodies, raised against monoclonal antibody OKM8, expressed determinants molecularly mimicking the CD36 binding domain for the adhesion of IRBC. Purified rabbit anti-Id antibodies reacted with the surface of IRBC by immunofluorescence, directly supported the adhesion of wild-type P. falciparum malaria isolates, and inhibited IRBC cytoadherence to melanoma cells. An approximately 270-kDa protein was immunoprecipitated by the anti-Id antibodies from surface-labeled and metabolically labeled IRBC and was competitively inhibited by soluble CD36. These results support the hypothesis that CD36 is a receptor and the approximately 270-kDa protein, sequestrin, is a complementary ligand involved in the adhesion of IRBC to host-cell endothelium. Sequestrin is a candidate malaria vaccine antigen, and anti-Id antibodies that recognize this molecule may be useful for passive immunotherapy of cerebral and severe P. falciparum malaria.