Aberrant expression of long noncoding RNAs in cumulus cells isolated from PCOS patients
Aberrant expression of long noncoding RNAs in cumulus cells isolated from PCOS patients
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PCOS 患者卵丘细胞中长非编码 RNA 的异常表达
DOI：
10.1007/s10815-015-0630-z
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发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Dai, Huangguan
中科院分区：
文献类型：
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作者：
Huang, Xin;Hao, Cuifang;Dai, Huangguan
目的应用基因芯片技术和生物信息学方法研究多囊卵巢综合征（PCOS）患者卵丘细胞中长链非编码RNA（lncRNA）的表达谱。这些信息将有助于我们了解PCOS的发生和发展。MethodsIn这项研究中，我们使用了一个芯片来描述从10例患者（5例PCOS和5例正常妇女）分离的卵丘细胞中的lncRNA谱。选择几种差异表达的lncRNA，通过定量RT-PCR（qRT-PCR）验证微阵列结果。然后，差异表达的lncRNA被分为三个亚组（HOX位点lncRNA，增强子样lncRNA，lincRNA），以推断其潜在的特征。此外，通过使用Cytoscape软件（V2.8.3， http://www.cytoscape.org/ 结果623条lncRNA和260条信使RNA（mRNA）表达显著上调或下调（变化≥2倍），这些差异可用于PCOS患者与正常人卵丘细胞的鉴别。选择5种差异表达的lncRNA（XLOC_011402、ENST 00000454271、ENST 00000433673、ENST 00000450294和ENST 00000432431），使用定量RT-PCR（qRT-PCR）验证微阵列结果。qRT-PCR结果与微阵列数据一致。进一步的分析表明，许多差异表达的lncRNA转录自2号染色体，并可能作为增强子调节其相邻的蛋白质编码基因。用43个lncRNA和29个mRNA构建了编码-非编码基因共表达网络。大多数对正相关，和一个mRNA相关的一个或多个lncRNA.ConclusionsOur研究是第一个确定全基因组lncRNA表达模式从PCOS患者分离的卵丘细胞的微阵列。结果表明，与正常妇女相比，PCOS患者卵丘细胞中存在着大量的lncRNA的异常表达，提示在PCOS患者和正常妇女卵丘细胞中存在lncRNA的差异表达，可能与PCOS的发生有关，并影响卵母细胞的发育。
PurposeTo describe the long noncoding RNA (lncRNA) profiles in cumulus cells isolated from polycystic ovary syndrome (PCOS) patients by employing a microarray and in-depth bioinformatics analysis. This information will help us understand the occurrence and development of PCOS.MethodsIn this study, we used a microarray to describe lncRNA profiles in cumulus cells isolated from ten patients (five PCOS and five normal women). Several differentially expressed lncRNAs were chosen to validate the microarray results by quantitative RT-PCR (qRT-PCR). Then, the differentially expressed lncRNAs were classified into three subgroups (HOX loci lncRNA, enhancer-like lncRNA, and lincRNA) to deduce their potential features. Furthermore, a lncRNA/mRNA co-expression network was constructed by using the Cytoscape software (V2.8.3, http://www.cytoscape.org/ ).ResultsWe observed that 623 lncRNAs and 260 messenger RNAs (mRNAs) were significantly up- or down-regulated (≥2-fold change), and these differences could be used to discriminate cumulus cells of PCOS from those of normal patients. Five differentially expressed lncRNAs (XLOC_011402, ENST00000454271, ENST00000433673, ENST00000450294, and ENST00000432431) were selected to validate the microarray results using quantitative RT-PCR (qRT-PCR). The qRT-PCR results were consistent with the microarray data. Further analysis indicated that many differentially expressed lncRNAs were transcribed from chromosome 2 and may act as enhancers to regulate their neighboring protein-coding genes. Forty-three lncRNAs and 29 mRNAs were used to construct the coding-non-coding gene co-expression network. Most pairs positively correlated, and one mRNA correlated with one or more lncRNAs.ConclusionsOur study is the first to determine genome-wide lncRNA expression patterns in cumulus cells isolated from PCOS patients by microarray. The results show that clusters of lncRNAs were aberrantly expressed in cumulus cells of PCOS patients compared with those of normal women, which revealed that lncRNAs differentially expressed in PCOS and normal women may contribute to the occurrence of PCOS and affect oocyte development.