Quantitative analysis of intravitreal injections in the rat
Quantitative analysis of intravitreal injections in the rat
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DOI：
10.1076/ceyr.22.1.74.6974
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发表时间：
2001-01-01
影响因子：
2
通讯作者：
Abitbol, M
中科院分区：
文献类型：
--
作者：
Dureau, P;Bonnel, S;Abitbol, M
玻璃体内注射目前在大鼠身上被用来在眼睛中引入一种治疗因子，特别是用于实验性的视网膜退化治疗。进样量及其位置会影响结果的量化。我们研究了一次玻璃体内注射对不同年龄和不同体积的大鼠的定量影响。3周龄或2月龄白化大鼠玻璃体腔注射中国墨汁1、3、5、10毫升。注射后立即处死动物，摘除眼球，固定，石蜡包埋，在矢状面进行显微切割。对规则间隔的切片进行分析以重建玻璃体和注入的染料体积。3周龄大鼠玻璃体体积为6.76±0.37 mm(3)，2个月龄大鼠玻璃体体积为13.36±0.64 mm(3)。注射后即刻玻璃体内墨水量分别为0.8 mm(3)、2 mm(3)、2.3~2.6 mm(3)和3.2 mm 3(10 Mul)。注射涉及的玻璃体体积百分比为4.4%~33.2%。注射体积受到大鼠晶状体大小的限制。注射体积越大，注射溶液的眼外损失越大，标准偏差越大。在这个模型中，染料倾向于定位在镜片后面。3或5个体积似乎具有最好的重现性和最小的溶液损失。
Intravitreal injections are currently used in the rat to introduce a therapeutic factor in the eye, especially for experimental treatments of retinal degenerations. The injected volume and its location can influence the quantification of results. We have investigated the quantitative effect of a single intravitreal injection in rats at different ages and for different volumes. Albinos rats aged three weeks or two months received intravitreal injections of 1, 3, 5 or 10 mul China ink. Animals were sacrificed immediately after injection, eyes were enucleated, fixated, embedded in paraffin and microtomy was performed in a sagittal plane. Regularly spaced sections were analyzed to reconstruct the vitreous and injected dye volumes. The measured vitreous volume was 6.76 +/- 0.37 mm(3) in three weeks old rats and 13.36 +/- 0.64 mm(3) in two months old rats. Mean intravitreal ink volumes immediately after injection were 0.8 mm(3) for 1 mul injections, 2 mm(3) for 3 mul, 2.3 to 2.6 mm(3) for 5 mul and 3.2 mm 3 for 10 mul. The percentage of vitreous volume involved by the injection ranged from 4.4% to 33.2%. The injected volume is limited by the large lens size of the rat. Extraocular loss of injected solution increases for higher injected volumes, with larger standard deviations. In this model, the dye tends to localize behind the lens. A 3 or 5 mul volume appears to have the best reproducibility with minimum loss of solution.