Identification of the rhizospheric microbe and metabolites that led by the continuous cropping of ramie (Boehmeria nivea L. Gaud).
Identification of the rhizospheric microbe and metabolites that led by the continuous cropping of ramie (Boehmeria nivea L. Gaud).
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连作苎麻 (Boehmeria nivea L. Gaud) 根际微生物及代谢产物的鉴定
DOI：
10.1038/s41598-020-77475-3
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发表时间：
2020-11-23
影响因子：
4.6
通讯作者：
Bai X
中科院分区：
文献类型：
--
作者：
Wang Y;Zhu S;Liu T;Guo B;Li F;Bai X
苎麻（Boehmeria nivea L.）Gaud）。本研究旨在揭示长期连作后健康植物和障碍植物宏基因组和代谢组的变化。经过10年的连作，苎麻种植在某些部分的土地表现出弱生长和低产量（障碍组），而苎麻种植在其他部分的土地生长健康（健康组）。我们收集了根际土壤和根样品，从土壤化学和植物理化性质的测量。对所有样品进行非靶向气相色谱-质谱（GS/MS）代谢组学分析。进一步利用宏基因组学技术对根际土壤生物的功能基因进行分析。结果表明，障碍组苎麻的株高、茎粗、纤维产量均低于健康组。此外，障碍群中根瘤菌科、溶杆菌属和慢生根瘤菌的相对丰度较低，而固氮菌属和固氮菌属的相对丰度较高。与健康组相比。代谢组学分析结果表明，半胱氨酰甘氨酸（Cys-Gly）、尿嘧啶、丙二酸和甘油是健康组和障碍组之间的关键差异代谢物。值得注意的是，这项工作揭示了细菌，如根瘤菌可能合成IAA，并可能减少苎麻的生物胁迫。L.在植物对抗生物胁迫方面，拟南芥也发挥积极作用，并由包括正磷酸盐、尿嘧啶和Cys-Gly的代谢物介导，这些代谢物可作为疾病风险的标志物。这些细菌效应可以通过代谢和甲硫氨酸代谢途径在植物抵抗生物胁迫中发挥关键作用。
Continuous cropping lowers the production and quality of ramie (Boehmeria nivea L. Gaud). This study aimed to reveal the metagenomic and metabolomic changes between the healthy- and obstacle-plant after a long period of continuous cropping. After 10 years of continuous cropping, ramie planted in some portions of the land exhibited weak growth and low yield (Obstacle-group), whereas, ramie planted in the other portion of the land grew healthy (Health-group). We collected rhizosphere soil and root samples from which measurements of soil chemical and plant physiochemical properties were taken. All samples were subjected to non-targeted gas chromatograph-mass spectrometer (GS/MS) metabolome analysis. Further, metagenomics was performed to analyze the functional genes in rhizospheric soil organisms. Based on the findings, ramie in Obstacle-group were characterized by shorter plant height, smaller stem diameter, and lower fiber production than that in Health-group. Besides, the Obstacle-group showed a lower relative abundance of Rhizobiaceae, Lysobacter antibioticus, and Bradyrhizobium japonicum, but a higher relative abundance of Azospirillum lipoferum and A. brasilense compared to the Health-group. Metabolomic analysis results implicated cysteinylglycine (Cys-Gly), uracil, malonate, and glycerol as the key differential metabolites between the Health- and Obstacle-group. Notably, this work revealed that bacteria such as Rhizobia potentially synthesize IAA and are likely to reduce the biotic stress of ramie. L. antibioticus also exerts a positive effect on plants in the fight against biotic stress and is mediated by metabolites including orthophosphate, uracil, and Cys-Gly, which may serve as markers for disease risk. These bacterial effects can play a key role in plant resistance to biotic stress via metabolic and methionine metabolism pathways.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4.9
作者：
Antoun, H;Beauchamp, CJ;Lalande, R
通讯作者：
Lalande, R
影响因子：
17.6
作者：
Li, Zhaoling;Chen, Jun;Wang, Zhong Lin
通讯作者：
Wang, Zhong Lin
影响因子：
5.8
作者：
Li, WZ;Jaroszewski, L;Godzik, A
通讯作者：
Godzik, A
影响因子：
3.6
作者：
Liu, Ming;Liu, Jia;Li, Zhongpei
通讯作者：
Li, Zhongpei