Carbon tetrachloride-induced lipid peroxidation and hyperglycemia in rat: A novel study
Carbon tetrachloride-induced lipid peroxidation and hyperglycemia in rat: A novel study
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DOI：
10.1177/0748233713475503
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发表时间：
2015-06-01
影响因子：
1.9
通讯作者：
Shah, Naseer Ali
中科院分区：
文献类型：
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作者：
Khan, Rahmat Ali;Khan, Muhammad Rashid;Shah, Naseer Ali
采用四氯化碳（CCl 4）诱导的大鼠胰腺氧化损伤和高血糖模型，研究了平卧龙葵甲醇提取物（LPME）的抗氧化作用。在CCl 4处理48小时后，向大鼠给予不同剂量的浸提液（3 ml/kg体重（bw）;腹腔注射，20% CCl 4/橄榄油），每周两次，持续4周。同时给予不同浓度的LPME（100，150和200 mg/kg）可改善CCl 4的毒性，并逆转血清酶（淀粉酶和脂肪酶），葡萄糖和激素（胰岛素）的水平。该提取物能降低胰腺组织中硫代巴比妥酸反应物，但增加谷胱甘肽含量。补充LPME可恢复CCl 4诱导的抗氧化酶活性（过氧化氢酶、过氧化物酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶和谷胱甘肽-S-还原酶）和DNA损伤。这表明，LPME有效地保护肝脏对四氯化碳诱导的氧化损伤，可能通过抗氧化和/或清除自由基的黄酮类化合物和酚类化合物的提取物的作用。
Launaea procumbens methanol extract (LPME) was evaluated against carbon tetrachloride (CCl4)-induced pancreatic oxidative damage and hyperglycemia in rats. Various doses of the extract were administered to rats after 48 h of CCl4 treatment (3 ml/kg bodyweight (bw); intraperitoneally, 20% CCl4/olive oil) twice a week for 4 weeks. Coadministration of various concentrations of LPME (100, 150 and 200 mg/kg) ameliorated the toxicity of CCl4 and reversed the serum level of enzymes (amylase and lipase), glucose and hormone (insulin). The extract was able to reduce thiobarbituric acid reactive substance but increased the glutathione contents in pancreatic tissue. Depletion of antioxidant enzyme activities (catalase, peroxidase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase and glutathione-S-reductase) and DNA damages induced with CCl4 were restored by LPME supplement. It is suggested that LPME effectively protects the liver against the CCl4-induced oxidative damage in rats, possibly through antioxidant and/or free radical scavenging effects of flavonoids and phenolic compounds in the extract.