Genetic organization of the she pathogenicity island in Shigella flexneri 2a
Genetic organization of the she pathogenicity island in Shigella flexneri 2a
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DOI：
10.1006/mpat.2000.0404
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发表时间：
2001-01-01
影响因子：
3.8
通讯作者：
Sakellaris, H
中科院分区：
文献类型：
--
作者：
Al-Hasani, K;Rajakumar, K;Sakellaris, H
本研究报道了福氏志贺菌2a株YSH 6000 T的致病岛（派）基因的全序列和遗传结构。she派为46 603 bp，位于pheV tRNA基因的3'端，在派的右边界含有pheV基因3'端22 bp的不完全直接重复序列。she派在派的pheV近端边界内携带噬菌体P4样整合酶基因、完整和截短的移动的遗传元件、质粒相关序列、开放阅读框，其显示出与在肠出血性大肠杆菌（EHEC）的肠上皮细胞消失（LEE）派和S. flexneri和其他几个未知功能的开放阅读框架。she派还编码两种自身转运蛋白，包括SigA，一种有助于肠液积聚的致细胞病变蛋白酶，和Pic，一种具有粘蛋白酶和血凝素活性的蛋白酶。另外，一个开放阅读框（orf）sap与编码抗原43的基因有很高的序列相似性，抗原43是大肠杆菌的一种表面自转运蛋白。杆菌ShET 1肠毒素基因主要与S.福氏2a菌株也位于she派上。(C)北京：科学出版社.
In this study we report the complete nucleotide sequence and genetic organization of the she pathogenicity island (PAI) of Shigella flexneri 2a strain YSH6000T. The 46 603 bp she PAI is situated adjacent to the 3' terminus of the pheV tRNA gene and includes an imperfect direct repeat of the 3'-terminal 22 bp of the pheV gene at the right boundary of the PAI. The she PAI carries a bacteriophage P4-like integrase gene within the pheV-proximal boundary of the PAI, intact and truncated mobile genetic elements, plasmid-related sequences, open reading frames exhibiting high sequence similarity to those found on the locus of enterocyte effacement (LEE) PAI of enterohemorrhagic Escherichia coli (EHEC), and the SHI-2 PAI of S. flexneri and several other open reading frames of unknown function. The she PAI also encodes two autotransporter proteins, including SigA, a cytopathic protease that contributes to intestinal fluid accumulation and Pic, a protease with mucinase, and hemagglutinin activities. In addition, an open reading frame (orf) termed sap, has high sequence similarity to the gene encoding Antigen 43, a surface-located autotransporter protein of E. coli. The ShET1 enterotoxin genes, associated predominantly with S. flexneri 2a strains, are also located on the she PAI. (C) 2001 Academic Press.