Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes: Implications in intestinal pathophysiology.
Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes: Implications in intestinal pathophysiology.
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DOI：
10.3748/wjg.v13.i47.6370
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发表时间：
2007-12
影响因子：
4.3
通讯作者：
P. Panigrahi;G. Braileanu;Hegang Chen;O. Stine
中科院分区：
文献类型：
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作者：
P. Panigrahi;G. Braileanu;Hegang Chen;O. Stine
目的研究大肠杆菌和益生菌感染Caco-2细胞后真核基因表达谱的变化。方法应用19,200个基因/表达序列标签的基因芯片检测正常植物群大肠杆菌感染Caco-2细胞后基因的表达。大肠杆菌、植物乳杆菌和两者的组合。结果基因芯片显示，大肠杆菌表达上调155个基因，下调177个基因。杆菌L. plantarum上调45个基因，下调36个基因。在混合感染过程中，27个基因表达上调，59个基因表达下调，大多数基因的刺激/抑制作用消失。在该组中观察到几个新基因的表达。结论本研究中所用的大肠杆菌菌株诱导了结肠样培养细胞中大量基因的表达，并改变了参与转录调控、蛋白质合成、代谢、细胞粘附、泛素化和凋亡等重要细胞过程的几个基因的表达。由益生菌的存在引起的这种变化可能会影响它们在宿主中引发的生理和病理反应。
AIM To investigate the change in eukaryotic gene expression profile in Caco-2 cells after infection with strains of Escherichia coli and commensal probiotic bacteria. METHODS A 19,200 gene/expressed sequence tag gene chip was used to examine expression of genes after infection of Caco-2 cells with strains of normal flora E. coli, Lactobacillus plantarum, and a combination of the two. RESULTS The cDNA microarray revealed up-regulation of 155 and down-regulation of 177 genes by E. coli. L. plantarum up-regulated 45 and down-regulated 36 genes. During mixed infection, 27 genes were up-regulated and 59 were down-regulated, with nullification of stimulatory/inhibitory effects on most of the genes. Expression of several new genes was noted in this group. CONCLUSION The commensal bacterial strains used in this study induced the expression of a large number of genes in colonocyte-like cultured cells and changed the expression of several genes involved in important cellular processes such as regulation of transcription, protein biosynthesis, metabolism, cell adhesion, ubiquitination, and apoptosis. Such changes induced by the presence of probiotic bacteria may shape the physiologic and pathologic responses they trigger in the host.