Changes in endotoxin-induced cytokine production by whole blood after in vivo exposure of normal humans to endotoxin.
Changes in endotoxin-induced cytokine production by whole blood after in vivo exposure of normal humans to endotoxin.
复制标题
正常人体内暴露于内毒素后，全血内毒素诱导的细胞因子产生的变化。
DOI：
10.1093/infdis/174.6.1356
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Lowry,SF
中科院分区：
文献类型：
--
作者：
vanderPoll,T;Coyle,SM;Moldawer,LL;Lowry,SF
6名健康受试者静脉注射脂多糖(LPS，2 ng/kg)，用10 ng/ml的LPS体外刺激全血。注射内毒素后3h和6h，全血产生的肿瘤坏死因子-α、白细胞介素1-Lβ、IL-6和IL-10均低于对照组(P均<0.05)。而注射脂多糖后，IL-L受体拮抗剂的产生明显增加(P<；0.05)。体内注射脂多糖后2 h的血浆对6例健康供者外周血中肿瘤坏死因子、IL-1β和IL-6的产生呈剂量依赖性抑制，而对IL-10和IL-L受体拮抗剂的产生无或仅有轻微的影响。内毒素耐受性代表了宿主的一种有目的的适应，而不是一种全身性低反应，至少部分是由先前暴露于内毒素后2小时内产生的可溶性因子介导的。
Whole blood of 6 healthy subjects, who were intravenously injected with lipopolysaccharide (LPS, 2 ng/kg), was stimulated ex vivo with LPS (10 ng/ml.). Three and 6 h after injection of LPS, whole blood produced less tumor necrosis factor-a (TNF), interleukin (lL)-lβ, IL-6, and IL-10 (allP< .05). By contrast, the production of IL-l receptor antagonist was enhanced after LPS injection (P< .05). Plasma obtained 2 h, but not 1 h, after in vivo administration of LPS showed a dosedependent inhibition of TNF, IL-1β, and IL-6 production by LPS-stimulated whole blood from 6 other healthy donors not previously exposed to LPS, while the production of IL-lO and IL-l receptor antagonist were not or were marginally influenced. LPS tolerance represents a purposeful adaptation of the host, rather than a generalized hyporesponsiveness, and is at least partly mediated by soluble factors produced within 2 h after previous exposure to LPS.