ELECTRON-MICROSCOPIC ANALYSIS OF THE NORMAL AND THE ACTIVATED PLEURAL MACROPHAGE
ELECTRON-MICROSCOPIC ANALYSIS OF THE NORMAL AND THE ACTIVATED PLEURAL MACROPHAGE
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DOI：
10.3109/01902149309064368
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发表时间：
1993-11-01
影响因子：
1.7
通讯作者：
STRANGE, C
中科院分区：
文献类型：
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作者：
BAUMANN, MH;HEINRICH, K;STRANGE, C
尽管在胸膜病理生理学中具有明显的作用，但有关胸膜巨噬细胞形态的信息知之甚少。胸膜内四环素（TCN）导致胸膜巨噬细胞流入和胸膜纤维化；胸膜内角叉菜胶（CAR）诱导巨噬细胞流入，但不会导致纤维化。用电子显微镜检查从正常 (NL) 和 TCN 或 CAR 暴露的兔胸膜腔收集的胸膜巨噬细胞。蜂窝尺寸；微绒毛数量；伪足；包被凹坑（CP）和包被囊泡（CV）；并测定了高尔基体、粗面内质网（RER）和中间丝（IF）的患病率。通过单因素方差分析评估每组中每个变量的均值，并通过Scheffe F检验进行post boc检验； p小于或等于0.5被认为是显着的。 TCN 刺激的胸膜巨噬细胞的特点是周长较小。 CAR诱导的胸膜巨噬细胞的特点是尺寸大且细胞内无定形物质丰富。与 TCN 诱导的或正常的巨噬细胞相比，它们具有更大的周长、面积和直径，因此每个面积的 CV + CP 数量更少。正常胸膜巨噬细胞的特征是比 CAR 或 TCN 诱导的胸膜巨噬细胞有更多的 IF、微绒毛和每周微绒毛。各组之间在核细胞质比率、伪足数量以及高尔基体或RER含量方面没有发现差异。结果表明，正常胸膜巨噬细胞与 TCN 和 CAR 诱导的胸膜巨噬细胞在形态上存在差异，并且这些形态差异反映了功能差异。
Despite an apparent role in pleural pathophysiology, little information is known about pleural macrophage morphology. Intrapleural tetracycline (TCN) results in pleural macrophage influx and pleural fibrosis; intrapleural carrageenan (CAR) induces macrophage influx without ensuing fibrosis. Pleural macrophages collected from normal (NL) and TCN- or CAR-exposed rabbit pleural spaces were examined with electron microscopy. Cellular size; number of microvilli; pseudopods; coated pits (CP) and coated vesicles (CV); and prevalence of golgi, rough endoplasmic reticulum (RER), and intermediate filaments (IF) were determined. The means of each variable in each group were assessed by one-way analysis of variance, with post boc testing performed by Scheffe F test; p less-than-or-equal-to 0.5 was considered significant. TCN-stimulated pleural macrophages were characterized by their small perimeters. CAR-induced pleural macrophages were marked by their large size and abundant intracellular amorphous material. They bad larger perimeters, areas, and diameters than the TCN-induced or normal macrophages and thus smaller numbers of CV + CP per area. The normal pleural macrophages were characterized by more IF, microvilli, and microvilli per perimeter than either the CAR- or TCN-induced pleural macrophages. No differences between groups were found in nuclear cytoplasmic ratios, number of pseudopods, and content of golgi or of RER. The results suggest that normal pleural macrophages and TCN- and CAR-induced pleural macrophages differ morphologically and that these morphologic differences reflect functional differences.