Role of Vascular Endothelial-Cadherin and p120-Catenin in the Formation o Experimental Intracranial Aneurysm in Animals
Role of Vascular Endothelial-Cadherin and p120-Catenin in the Formation o Experimental Intracranial Aneurysm in Animals
复制标题
血管内皮细胞钙粘蛋白和 p120-连环蛋白在动物实验性颅内动脉瘤形成中的作用
DOI：
10.1016/j.wneu.2019.04.077
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发表时间：
2019-08-01
影响因子：
2
通讯作者：
Li，Mei-hua
中科院分区：
文献类型：
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作者：
Xiao，Zhi-peng;Zhao，Jian-lan;Li，Mei-hua
背景内皮细胞（ECs）功能障碍是颅内动脉瘤（IAs）形成的关键因素。然而，对ECs对血流动力学损伤的反应及其对IA形成的贡献知之甚少。方法用成年雌性新西兰大白兔和雄性sd大鼠分别建立sias模型。苏木精染色、伊红染色检测血管壁形态变化。荧光染色分析动脉分叉正中矢状面p120-catenin （p120ctn）和血管内皮-钙粘蛋白的分子和细胞变化。结果实验兔颅底端及大鼠大脑前动脉-嗅动脉分叉处均可见破坏性动脉瘤重构及形态IAs的形成。与血管内皮-钙粘蛋白共定位的p120ctn在ECs中的表达降低。此外，与ECs细胞核共定位的p120ctn表达增加。这些事件表明p120ctn从膜转运到ECs的细胞核。结论不同的血流动力学条件可诱发血管壁内皮损伤，其潜在机制可能是血管内皮损伤总是发生在分叉尖。动脉分叉处的血流动力学改变可能引发IAs的形成。
BackgroundDysfunction of endothelial cells (ECs) constitutes a critical factor in the formation of intracranial aneurysms (IAs). However, little is known about the response of ECs to hemodynamic insults and its contribution to IA formation.MethodsIAs models were constructed in both adult female New Zealand white rabbits and male Sprague–Dawley rats. Morphologic changes of vessel wall were detected by hematoxylin and eosin staining. Molecular and cellular changes, including p120-catenin (p120ctn) and vascular endothelial−cadherin, in the median sagittal section of the artery bifurcation were analyzed by fluorescent staining.ResultsDestructive aneurysmal remodeling and the formation of morphologic IAs were observed at the basilar termini of experimental rabbits and the anterior cerebral artery–olfactory artery bifurcation of rats. The expression of p120ctn colocalized with vascular endothelial−cadherin in ECs decreased. Moreover, the expression of p120ctn colocalized with nucleus of ECs increased. These events suggested that p120ctn was transported from the membrane to the nucleus of ECs.ConclusionsThe potential mechanism, that IAs are always localizing in the bifurcation apices, may be that the endothelium injury of vessel wall can be induced by different hemodynamic conditions. Hemodynamic changes in artery bifurcation may initiate the formation of IAs.