Osteopontin might be involved in bone remodelling rather than in inflammation in ankylosing spondylitis
Osteopontin might be involved in bone remodelling rather than in inflammation in ankylosing spondylitis
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DOI：
10.1093/rheumatology/ken385
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发表时间：
2008-12-01
期刊：
影响因子：
5.5
通讯作者：
Lee, S. -K.
中科院分区：
文献类型：
--
作者：
Choi, S. T.;Kim, J. H.;Lee, S. -K.
目标。目的：探讨骨桥蛋白（OPN）在AS患者中是否升高，并探讨其与炎性疾病活动性和骨重构过程的关系。这项横断面研究包括30名AS患者和23名年龄和性别匹配的健康对照。我们评估了临床特征和实验室参数，包括ESR、CRP、脂质谱、巴斯AS疾病活动指数（BASDAI）和巴斯AS放射学指数（BASRI）。为了评估骨代谢，我们检测了ALP、OCN和I型胶原的c -末端肽（CTX-I）。ELISA法检测血浆OPN、TNF-、IL-6水平，RTPCR法检测外周血单个核细胞mRNA表达。在接受TNF-阻滞剂治疗后，对8例患者的OPN水平变化进行了评估。AS患者的血浆OPN、TNF-和IL-6水平明显高于健康对照组，mRNA表达也明显高于健康对照组。血浆OPN水平与血清ALP、OCN和CTX-I水平相关，但与ESR、CRP、脂质谱、BASDAI或BASRI无关。TNF-阻滞剂治疗不改变OPN水平，尽管它降低了疾病活动性。与对照组相比，AS患者的OPN水平更高。血浆OPN水平与血清ALP、OCN和CTX-I水平相关，但与AS患者的疾病活动性无关。OPN可能参与AS的骨重塑而非炎症。
Objectives. To determine whether osteopontin (OPN) is increased in patients with AS and to investigate its relationship to inflammatory disease activity and bone remodelling process.Methods. This cross-sectional study included 30 patients with AS and 23 age- and sex-matched healthy controls. We assessed clinical characteristics and laboratory parameters including the ESR, CRP, lipid profiles, the Bath AS disease activity index (BASDAI) and the Bath AS radiographic index (BASRI). To evaluate bone metabolism, we tested ALP, OCN and C-telopeptide of type I collagen (CTX-I). Plasma levels of OPN, TNF- and IL-6 were measured by ELISA, and mRNA expression in peripheral blood mononuclear cells (PBMCs) was performed by RTPCR. Changes in OPN level were also evaluated in eight patients after the treatment with a TNF- blocker.Results. Patients with AS had significantly higher plasma OPN, TNF- and IL-6 levels and more mRNA expression than healthy controls. Plasma OPN levels were correlated with serum ALP, OCN and CTX-I levels, but not with ESR, CRP, lipid profiles, BASDAI or BASRI. Treatment with a TNF- blocker did not alter OPN levels, although it reduced the disease activity.Conclusions. Patients with AS had higher levels of OPN compared with controls. The plasma OPN level was correlated with serum ALP, OCN and CTX-I levels, but not with disease activity in AS. OPN might be involved in bone remodelling rather than in inflammation in AS.