A fluorescence-based optical fiber analyzer for catecholamine determination
A fluorescence-based optical fiber analyzer for catecholamine determination
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DOI：
10.1039/c2ay25076d
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发表时间：
2012-07
影响因子：
3.1
通讯作者：
Lurdes I. B. Silva;K. Duarte;A. Freitas;T. Panteleitchouk;T. Rocha-Santos;M. E. Pereira;A. Duarte
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文献类型：
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作者：
Lurdes I. B. Silva;K. Duarte;A. Freitas;T. Panteleitchouk;T. Rocha-Santos;M. E. Pereira;A. Duarte
研制了一种用于生物样品中儿茶酚胺(多巴胺、去甲肾上腺素和肾上腺素)诱导荧光分析的光纤分析仪。分析装置包括儿茶酚胺分离色谱柱和基于荧光的检测装置(FOF分析仪)。FOF分析仪的检出限小于0.9pg/L−1。该方法可用于实际尿样中儿茶酚胺的测定。采用高效液相色谱-电化学检测法对FOF-儿茶酚胺分析仪的分析性能进行了考察。与高效液相色谱-电泳法和其他方法如高效液相-荧光法相比，FOF-分析仪对多巴胺(DA)、去甲肾上腺素(NE)和肾上腺素(E)的检测下限较低，线性范围较大。这些优点与紧凑的设计、小规模的仪器设备和有效的分析成本相结合，使该系统成为现有方法测定临床样品中儿茶酚胺的有趣替代方案。
An optical fibre (OF) analyzer for measuring catecholamines (dopamine, norepinephrine and epinephrine) in biological samples with induced fluorescence was developed. The analytical set-up included a chromatographic column for catecholamine separation and a fluorescence-based OF detection (FOF-analyzer). The detection limit of the FOF-analyser was found to be less than 0.9 pg mL−1. The proposed methodology showed an adequate analytical performance for the determination of the catecholamines in actual samples of human urine. The analytical performance of the FOF-analyzer for catecholamine determination was investigated against the high performance liquid chromatography-electrochemical detection (HPLC-ED) method. The FOF-analyzer showed lower detection limits and larger linear ranges for determination of dopamine (DA), norepinephrine (NE) and epinephrine (E) in comparison with HPLC-ED and other methodologies such as HPLC-fluorescence. These advantages combined with the compact design, small-scale instrumentation, and effective cost of analysis make this system an interesting alternative to the existing methodologies for the determination of catecholamines in clinical samples.