Controlled cyclic stretch bioreactor for tissue-engineered heart valves
Controlled cyclic stretch bioreactor for tissue-engineered heart valves
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DOI：
10.1016/j.biomaterials.2009.04.027
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发表时间：
2009-09-01
期刊：
影响因子：
14
通讯作者：
Tranquillo, Robert T.
中科院分区：
文献类型：
--
作者：
Syedain, Zeeshan H.;Tranquillo, Robert T.
组织工程心脏瓣膜(TEHV)代表着最终的瓣膜置换，特别是对于青少年患者来说，考虑到它的生长潜力。到目前为止，大多数TEHV生物反应器的开发都是基于培养介质通过瓣膜腔的脉冲流来在小叶中诱导应变。使用先前报道的管状结构的受控循环拉伸策略，我们开发了一种用于TEHs的受控循环拉伸生物反应器，从而改善了拉伸和成分性能。TEHV安装在乳胶管内，然后用培养基循环加压。根部和小叶的伸展与乳胶相称，伸展是由较硬的乳胶决定的，因此是可控的。在流环中，介质也以缓慢的速度流过管腔，以提供营养输送。以人真皮成纤维细胞制备的纤维蛋白为基础的TEHV在三周的循环拉伸下，应变幅值逐渐增加。TEHV具有绵羊肺动脉瓣叶的拉伸硬度和硬度各向异性，并能承受与绵羊肺动脉相似的周期性肺压力。(C)2009爱思唯尔有限公司。保留所有权利。
A tissue-engineered heart valve (TEHV) represents the ultimate valve replacement, especially for juvenile patients given its growth potential. To date, most TEHV bioreactors have been developed based on pulsed flow of culture medium through the valve lumen to induce strain in the leaflets. Using a strategy for controlled cyclic stretching of tubular constructs reported previously, we developed a controlled cyclic stretch bioreactor for TEHVs that leads to improved tensile and compositional properties. The TEHV is mounted inside a latex tube, which is then cyclically pressurized with culture medium. The root and leaflets stretch commensurately with the latex, the stretching being dictated by the stiffer latex and thus controllable. Medium is also perfused through the lumen at a slow rate in a flow loop to provide nutrient delivery. Fibrin-based TEHVs prepared with human dermal fibroblasts were subjected to three weeks of cyclic stretching with incrementally increasing strain amplitude. The TEHV possessed the tensile stiffness and stiffness anisotropy of leaflets from sheep pulmonary valves and could withstand cyclic pulmonary pressures with similar distension as for a sheep pulmonary artery. (C) 2009 Elsevier Ltd. All rights reserved.