MANGANESE+2 EXHIBITS DYNAMIC BINDING TO MULTIPLE LIGANDS IN HUMAN PLASMA
MANGANESE+2 EXHIBITS DYNAMIC BINDING TO MULTIPLE LIGANDS IN HUMAN PLASMA
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DOI：
10.1016/0026-0495(92)90290-q
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发表时间：
1992-10-01
影响因子：
9.8
通讯作者：
KEEN, CL
中科院分区：
文献类型：
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作者：
CRITCHFIELD, JW;KEEN, CL
用54mncl2体外标记禁食成年男性受试者的血浆，然后使用几种技术进行分离。分子筛色谱分析表明，主要的含54mn的峰具有非常低的分子量（VLMW），尽管也观察到另外四个显著的峰，其中一个峰对应转铁蛋白（Tf）的质量。随着体外培养时间的延长，Tf峰的54mn含量增加，表明Mn+2在与Tf结合之前已经氧化为Mn+3。这种时间依赖性效应通过由固定的anti-Tf组成的亲和层析来验证。等离子体的电泳分析产生了模棱两可的结果，表明这种方法在研究等离子体锰定位方面的价值有限。上述研究结果在影响人体血浆中Mn2+氧化和代谢的因素的背景下进行了讨论。
Plasma from fasted adult male subjects was labeled in vitro with54MnCl2and then fractionated using several techniques. Molecular sieve chromatography showed that the major54Mn-containing peak had a very low molecular weight (VLMW), although four other significant peaks, one of which corresponded to the mass of transferrin (Tf), were also observed. The54Mn content of the Tf peak increased with increasing incubation time in vitro, suggesting the oxidation of Mn+2to Mn+3before its association with Tf. This time-dependent effect was verified using affinity chromatography consisting of immobilized anti-Tf. Electrophoretic analyses of plasma yielded equivocal results, indicating a limited value of this method for investigating plasma manganese localization. The above findings are discussed in the context of factors that influence the oxidation and metabolism of Mn2+in human plasma.