Capacity of the Golgi apparatus for cargo transport prior to complete assembly
Capacity of the Golgi apparatus for cargo transport prior to complete assembly
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DOI：
10.1091/mbc.e05-12-1112
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发表时间：
2006-09-01
影响因子：
3.3
通讯作者：
Storrie, Brian
中科院分区：
文献类型：
--
作者：
Jiang, Shu;Rhee, Sung W.;Storrie, Brian
在酵母中，特别强调内质网（ER）衍生的高尔基体组装的池成熟模型，而在哺乳动物细胞中，更强调高尔基蛋白作为潜在的稳定组装框架。在 HeLa 细胞中布雷菲德菌素 A/H89 洗脱后，内质网从头形成高尔基体的情况下，我们发现分散的、富含高尔基体的结构早期形成，并含有高尔基体，包括巨蛋白，范围横跨高尔基体的整个顺式到反式谱。这些结构无法胜任 VSV-G 货物运输。其次，我们将高尔基体在货物运输中的能力与药物冲洗后将各种糖基转移酶和糖苷酶添加到新生的、富含高尔金的结构中的动力学进行了比较。在分散的新生高尔基体中，酶的积累与反式糖基转移酶/糖苷酶依次积累，然后是中间糖基转移酶/糖苷酶。在酶或 GPP130 完全积累到新生高尔基体之前，就参与了货物运输。第三，在有丝分裂期间，我们发现在末期早期高尔金阳性受体区室的形成先于高尔基糖基转移酶在新生高尔基结构中的积累。我们得出的结论是，在哺乳动物高尔基体组装过程中，组件装入动态的、首先形成的、富含多高尔金的框架中，该框架最初是无能力进行货物运输的。在高尔基体完全组装之前恢复货物运输。
In yeast, particular emphasis has been given to endoplasmic reticulum (ER)-derived, cisternal maturation models of Golgi assembly while in mammalian cells more emphasis has been given to golgins as a potentially stable assembly framework. In the case of de novo Golgi formation from the ER after brefeldin A/H89 washout in HeLa cells, we found that scattered, golgin-enriched, structures formed early and contained golgins including giantin, ranging across the entire cis to trans spectrum of the Golgi apparatus. These structures were incompetent in VSV-G cargo transport. Second, we compared Golgi competence in cargo transport to the kinetics of addition of various glycosyltransferases and glycosidases into nascent, golgin-enriched structures after drug washout. Enzyme accumulation was sequential with trans and then medial glycosyltransferases/glycosidases found in the scattered, nascent Golgi. Involvement in cargo transport preceded full accumulation of enzymes or GPP130 into nascent Golgi. Third, during mitosis, we found that the formation of a golgin-positive acceptor compartment in early telophase preceded the accumulation of a Golgi glycosyltransferase in nascent Golgi structures. We conclude that during mammalian Golgi assembly components fit into a dynamic, first-formed, multigolgin-enriched framework that is initially cargo transport incompetent. Resumption of cargo transport precedes full Golgi assembly.