ACCUMULATION OF GLYCINEBETAINE DURING COLD-ACCLIMATION AND FREEZING TOLERANCE IN LEAVES OF WINTER AND SPRING BARLEY PLANTS
ACCUMULATION OF GLYCINEBETAINE DURING COLD-ACCLIMATION AND FREEZING TOLERANCE IN LEAVES OF WINTER AND SPRING BARLEY PLANTS
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DOI：
10.1111/j.1365-3040.1994.tb00269.x
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发表时间：
1994-01-01
影响因子：
7.3
通讯作者：
TAKABE, T
中科院分区：
文献类型：
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作者：
KISHITANI, S;WATANABE, K;TAKABE, T
一项研究旨在研究甜菜碱(甜菜碱)，一种亲和的溶质，是否在冷胁迫下积累，并参与保护植物免受冻害的机制。为此，我们使用了大麦的近等基因系，每个系只在春季类型的生长习性的单个基因上不同；这些不同的系是通过与冬型轮回品种的回交产生的。冬季类型的生长习性需要较低的温度来触发花的发育(春化)，而春季类型则不需要。在3周的低温(5℃)条件下，冬性品系和春性生长习性为sh基因的春-Sh品系甜菜碱积累水平为基础水平的5倍，而春性生长习性为Sh(3)基因的春系-Sh(3)品系甜菜碱含量仅为基础水平的两倍。在同一品种的近等基因系中，叶片在低温下积累的甜菜碱水平与绿叶在-5℃冻害中存活的百分比(以干重为基础)之间存在很好的相关性。这一观察结果表明，除了甜菜碱在盐分和/或干旱反应中的已知作用外，甜菜碱在低温胁迫下积累的可能性也是可能的，而且在低温驯化过程中甜菜碱的积累在一定程度上与大麦叶片的耐寒性有关。
A study was performed to examine whether or not betaine (glycinebetaine), a compatible solute, is accumulated in response to cold stress and is involved in mechanisms that protect plants from freezing injury. For this purpose, we used near-isogenic lines of barley, with each line differing only in a single gene for the spring type of growth habit; the various lines were produced by back-crosses to a recurrent cultivar of the winter type. The winter type of growth habit requires a low temperature for triggering of flower development (vernalization), whereas the spring type does not. Betaine was accumulated to five times the basal level over the course of 3 weeks at low temperature (5 degrees C) in the winter-type cultivar and in a spring-sh line having the sh gene for the spring-type growth habit, but the level was only doubled in the spring-Sh(3) line, which carried the Sh(3) gene for the spring-type growth habit. Among near-isogenic lines of the same cultivar, the levels of betaine accumulated in leaves at low temperature were well correlated with the percentages (on a dry weight basis) of green leaves that survived freezing injury (-5 degrees C). This observation indicates the possibility, separate from the recognized role of betaine in the response to salinity and/or drought, that betaine accumulates in response to cold stress and that the accumulation of betaine during cold acclimation is associated to some extent with freezing tolerance in leaves of barley plants.