Effect of local bone marrow stromal cell administration on ligature-induced periodontitis in mice
Effect of local bone marrow stromal cell administration on ligature-induced periodontitis in mice
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DOI：
10.2334/josnusd.16-0033
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发表时间：
2017-12-01
影响因子：
1.9
通讯作者：
Honda, Masaki
中科院分区：
文献类型：
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作者：
Iguchi, Shinya;Suzuki, Daigo;Honda, Masaki
骨髓来源的多能基质细胞（BMSCs）具有强大的造血功能。本研究旨在探讨骨髓间充质干细胞在小鼠结扎性牙周炎模型中的修复潜力。从小鼠的股骨和胫骨分离BMSC。牙周炎是通过在右上颌第二磨牙周围放置结扎线来诱导的。3天后，在结扎的磨牙周围的近中牙间乳头的牙龈中向小鼠施用BMSC。未给予BMSC的结扎和未结扎小鼠作为对照。第二磨牙牙根周围炎症浸润和骨吸收的差异进行了评估，随后使用显微计算机断层扫描（micro-CT），组织学分析和抗酒石酸酸性磷酸酶（TRAP）染色进行定量。Micro-CT显示，结扎磨牙周围的牙槽骨丢失以时间依赖性的方式增加;然而，与结扎对照小鼠相比，BMSC治疗小鼠的效果显著降低。组织病理学显示，BMSC给药减轻了结扎BMSC小鼠的炎症浸润。此外，TRAP阳性破骨细胞的数量在结扎对照组小鼠与BMSC治疗组小鼠相比显著升高。这些发现表明，局部BMSC给药可以减轻炎症和牙槽骨吸收，表明给药BMSC导致牙周炎的新疗法。
Bone marrow-derived multipotent stromal cells (BMSCs) have potent antiinflammatory effects. This study aimed to investigate the antiinflammatory potential of BMSCs using a mouse model of ligature-induced periodontitis. BMSCs were isolated from the femurs and tibiae of mice. Periodontitis was induced by placing a ligature around the right maxillary second molar. After 3 days, the mice were administered BMSC in the gingiva of the mesial interdental papilla around the ligatured molar. The ligatured and non-ligatured mice that were not administered BMSC served as controls. Differences in inflammatory infiltration and bone resorption around the roots of the second molar were assessed and were subsequently quantified using microcomputed tomography (micro-CT), histological analysis, and tartrate-resistant acid phosphatase (TRAP) staining. Micro-CT revealed that alveolar bone loss around the ligatured molars increased in a time-dependent manner; however, the effect was significantly less in BMSC-treated mice compared with ligatured control mice. Tissue histopathology revealed that BMSC administration mitigated inflammatory infiltration in ligatured BMSC mice. In addition, the number of TRAP-positive osteoclasts was markedly elevated in ligatured control mice compared with those in BMSC-treated mice. These findings indicate that local BMSC administration can mitigate inflammation and alveolar bone resorption, suggesting that administering BMSC leads to new therapeutics for periodontitis.