IMPROVED DETECTION AND CHARACTERIZATION OF FUCOXANTHIN‐TYPE CAROTENOIDS: NOVEL PIGMENTS IN EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) 1
IMPROVED DETECTION AND CHARACTERIZATION OF FUCOXANTHIN‐TYPE CAROTENOIDS: NOVEL PIGMENTS IN EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) 1
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DOI：
10.1111/j.1529-8817.2006.00199.x
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发表时间：
2006-03
影响因子：
2.9
通讯作者：
R. Airs;C. Llewellyn
中科院分区：
文献类型：
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作者：
R. Airs;C. Llewellyn
一种新的大气压化学电离质谱（APCI‐LC/MS）方法改进了岩藻黄素相关类胡萝卜素的检测和辅助表征，揭示了球石藻Emiliania huxleyi（Lohm.）Hay et Mohler（菌株MBA 92，Plymouth）含有的酰氧基岩藻黄质范围比以前报道的更广。这种多样性被证实是由在C-19′位酯化的链烷酸取代基的长度差异引起的。在Emiliania huxleyi的培养物中检测到了在C-19′位具有4 - 8个碳取代基的酰氧基岩藻黄质，其中以前文献中仅报道了19′-丁酰氧基岩藻黄质和19′-己酰氧基岩藻黄质。在Emiliania huxleyi中检测到这些新的类胡萝卜素，可以详细研究环境因素对该生物体中岩藻黄素型类胡萝卜素复杂库中单个组分的影响。
A new atmospheric pressure chemical ionization mass spectrometry (APCI‐LC/MS) method improved detection and aided characterization of fucoxanthin related carotenoids, revealing the coccolithophorid Emiliania huxleyi (Lohm.) Hay et Mohler (strain MBA 92, Plymouth) to contain a wider range of acyloxyfucoxanthins than reported previously. The diversity is confirmed as arising from differences in the length of the alkanoic acid substituent esterified at position C‐19′. Acyloxyfucoxanthins with substituents of between four and eight carbons at the C‐19′ position have been detected in a culture of Emiliania huxleyi, where previously only 19′‐butanoyloxyfucoxanthin and 19′‐hexanoyloxyfucoxanthin have been reported in the literature. Novel fucoxanthinol derivatives were also found. The detection of these novel carotenoids in Emiliania huxleyi permits detailed studies of the impact of environmental factors on individual components of the complex pool of fucoxanthin‐type carotenoids in this organism.