The flanking region sequences of the 15-kDa lipoprotein gene differentiate pathogenic treponemes
The flanking region sequences of the 15-kDa lipoprotein gene differentiate pathogenic treponemes
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DOI：
10.1086/515247
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发表时间：
1998-04-01
影响因子：
6.4
通讯作者：
Lukehart, SA
中科院分区：
文献类型：
--
作者：
Centurion-Lara, A;Castro, C;Lukehart, SA
梅毒螺旋体包括三个亚种（苍白螺旋体、细小螺旋体和地方螺旋体），分别引起梅毒、雅司病和贝杰尔病。一个密切相关的物种，梅毒螺旋体，是性病梅毒的病原体在兔子，但不感染人类，虽然这些螺旋体引起不同的疾病，没有实验室方法的区分已被报道。在15-kDa脂蛋白基因（tpp 15）的5'和3'侧翼区定义了区分人类病原体和T. paraluiscuniculi，以及区分T.梅毒螺旋体亚种（T. pallidum subsp），来自人类非性病性密螺旋体病的梅毒螺旋体。苍白球亚种pallidum与其他亚种和种不同，3'侧翼区的XcmI位点将T.从人类病原体中分离出来的。应用聚合酶链反应和限制性酶切多态性技术对27株致病性密螺旋体进行分析。
The species Treponema pallidum includes three subspecies (pallidum, pertenue, and endemicum) that cause syphilis, yaws, and bejel, respectively. A closely related species, Treponema paraluiscuniculi, is the etiologic agent of venereal syphilis in rabbits but does not infect humans, Although these treponemes cause distinct diseases, no laboratory method for differentiation has been reported. Genetic signatures were defined in the 5' and 3' flanking regions of the 15-kDa lipoprotein gene (tpp15) that distinguish the human pathogens and T. paraluiscuniculi, as well as distinguishing T. pallidum subsp, pallidum from the causes of human nonvenereal treponematoses, A single Eco47III restriction site in the 5' flanking region differentiates T. pallidum subsp. pallidum from the other subspecies and species, and an XcmI site in the 3' flanking region differentiates T. paraluiscuniculi from the human pathogens. Polymerase chain reaction methods and restriction polymorphism were used to analyze 27 strains of pathogenic Treponema species.