The role of effector molecules in signal transduction by PII proteins
The role of effector molecules in signal transduction by PII proteins
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DOI：
10.1042/bst0390189
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发表时间：
2011-02-01
影响因子：
3.9
通讯作者：
Merrick, Mike
中科院分区：
文献类型：
--
作者：
Radchenko, Martha;Merrick, Mike
P-II蛋白是自然界中分布最广泛的信号转导蛋白之一，广泛存在于细菌、古细菌和植物中。它们通过蛋白质-蛋白质相互作用来控制各种酶、转录因子和转运蛋白的活性，其中绝大多数参与细胞氮代谢。P-II蛋白的调节活性通过其结合关键效应代谢物2-OG（2-酮戊二酸）、ATP和ADP的能力介导。然而，这些调节作用的分子基础仍不清楚。最近的进展，在解决1311蛋白质的晶体结构与他们的一些目标蛋白质，以及识别的ATP/ADP-和2-OG-结合位点，提高了我们的理解，他们的行动模式。在迄今为止解决的所有复杂结构中，1311的柔性T环促进了与靶蛋白的相互作用。效应分子似乎在调节T环的构象中起关键作用，从而调节P-II与其靶标之间的相互作用。
P-II proteins are one of the most widely distributed signal transduction proteins in Nature, being ubiquitous in bacteria, archaea and plants. They act by protein-protein interaction to control the activities of a wide range of enzymes, transcription factors and transport proteins, the great majority of which are involved in cellular nitrogen metabolism. The regulatory activities of P-II proteins are mediated through their ability to bind the key effector metabolites 2-OG (2-oxoglutarate), ATP and ADP. However, the molecular basis of these regulatory effects remains unclear. Recent advances in the solution of the crystal structures of 1311 proteins complexed with some of their target proteins, as well as the identification of the ATP/ADP- and 2-OG-binding sites, have improved our understanding of their mode of action. In all of the complex structures solved to date, the flexible T-loops of 1311 facilitate interaction with the target protein. The effector molecules appear to play a key role in modulating the conformation of the T-loops and thereby regulating the interactions between P-II and its targets.