Induction of Spontaneous Hyaline Cartilage Regeneration Using a Double-Network Gel Efficacy of a Novel Therapeutic Strategy for an Articular Cartilage Defect
Induction of Spontaneous Hyaline Cartilage Regeneration Using a Double-Network Gel Efficacy of a Novel Therapeutic Strategy for an Articular Cartilage Defect
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DOI：
10.1177/0363546511399383
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发表时间：
2011-06-01
影响因子：
4.8
通讯作者：
Gong, Jian Ping
中科院分区：
文献类型：
--
作者：
Kitamura, Nobuto;Yasuda, Kazunori;Gong, Jian Ping
背景：由聚(2-丙烯酰胺基-2-甲基丙磺酸)和聚(N，N‘-二甲基丙烯酰胺)(PAMPS/PDMAAm)组成的双网络凝胶在体外和体内均具有诱导软骨形成的潜能。目的：建立一种应用双网络凝胶治疗关节软骨缺损的疗效。在右膝关节的缺损处植入一个DN凝胶塞子，使术后留下2 mm深的缺损区。一个未经治疗的左膝缺陷提供了对照数据。术后4周和12周分别进行组织学和免疫组织化学评价、激光共聚焦扫描显微镜表面评价和实时聚合酶链式反应(PCR)分析。用Wayne等人和O‘Driscoll等人报道的2种评分系统对样本进行定量评价。结果：移植的骨缺损处充填了足够体积的富含蛋白多糖和2型胶原的透明软骨组织。用分级量表进行定量评估，结果显示，在各个时期，植入的牙周膜凝胶与未经处理的对照组相比，其得分显著升高(P
Background: A double-network (DN) gel, which was composed of poly-(2-acrylamido-2-methylpropanesulfonic acid) and poly(N, N'-dimetyl acrylamide) (PAMPS/PDMAAm), has the potential to induce chondrogenesis both in vitro and in vivo.Purpose: To establish the efficacy of a therapeutic strategy for an articular cartilage defect using a DN gel.Study Design: Controlled laboratory study.Methods: A 4.3-mm-diameter osteochondral defect was created in rabbit trochlea. A DN gel plug was implanted into the defect of the right knee so that a defect 2 mm in depth remained after surgery. An untreated defect of the left knee provided control data. The osteochondral defects created were examined by histological and immunohistochemical evaluations, surface assessment using confocal laser scanning microscopy, and real-time polymerase chain reaction (PCR) analysis at 4 and 12 weeks. Samples were quantitatively evaluated with 2 scoring systems reported by Wayne et al and O'Driscoll et al.Results: The DN gel-implanted defect was filled with a sufficient volume of the hyaline cartilage tissue rich in proteoglycan and type 2 collagen. Quantitative evaluation using the grading scales revealed a significantly higher score in the DN gel-implanted defects compared with the untreated control at each period (P