Bioactivity and biomineralization ability of calcium silicate-based pulp-capping materials after subcutaneous implantation
Bioactivity and biomineralization ability of calcium silicate-based pulp-capping materials after subcutaneous implantation
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DOI：
10.1111/iej.12802
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发表时间：
2017-12-01
影响因子：
5
通讯作者：
Okiji, T.
中科院分区：
文献类型：
--
作者：
Hinata, G.;Yoshiba, K.;Okiji, T.
目的评价三种硅酸钙基盖髓材料（ProRoot MTA、TheraCal LC和原型硅酸三钙水泥）皮下植入大鼠后产生磷灰石样沉淀的能力。方法将装有每种材料的聚四氟乙烯管皮下植入Wistar大鼠背部。植入后7、14和28天，将植入物与周围的结缔组织一起取出，并固定在含2.5%戊二醛的二甲胂酸盐缓冲液中。使用扫描电子显微镜-电子探针微量分析（SEM-EPMA）分析植入物上形成的表面沉淀物的化学成分。还使用 SEM-EPMA 分析了材料-组织界面处钙 (Ca) 和磷 (P) 的分布。使用弗里德曼检验和 Scheffe 检验对富钙和磷区域的厚度进行比较，显着性水平为 5%。结果所有三种材料均产生含有钙和磷的磷灰石状表面沉淀物。对于每种材料，元素图检测到结缔组织区域，其中钙和磷的浓度高于周围结缔组织中的浓度。该富含钙和磷的区域的厚度表现出以下模式：ProRoot MTA > 原型硅酸三钙水泥TheraCal LC。 ProRoot MTA 在所有时间点都比其他材料具有明显更厚的 Ca 和 P 层 (P
AimTo evaluate the abilities of three calcium silicate-based pulp-capping materials (ProRoot MTA, TheraCal LC and a prototype tricalcium silicate cement) to produce apatite-like precipitates after being subcutaneously implanted into rats.MethodologyPolytetrafluoroethylene tubes containing each material were subcutaneously implanted into the backs of Wistar rats. At 7, 14 and 28days post-implantation, the implants were removed together with the surrounding connective tissue, and fixed in 2.5% glutaraldehyde in cacodylate buffer. The chemical compositions of the surface precipitates formed on the implants were analysed with scanning electron microscopy-electron probe microanalysis (SEM-EPMA). The distributions of calcium (Ca) and phosphorus (P) at the material-tissue interface were also analysed with SEM-EPMA. Comparisons of the thicknesses of the Ca- and P-rich areas were performed using the Friedman test followed by Scheffe's test at a significant level of 5%.ResultsAll three materials produced apatite-like surface precipitates containing Ca and P. For each material, elemental mapping detected a region of connective tissue in which the concentrations of Ca and P were higher than those in the surrounding connective tissue. The thickness of this Ca- and P-rich region exhibited the following pattern: ProRoot MTA > prototype tricalcium silicate cementTheraCal LC. ProRoot MTA had a significantly thicker layer of Ca and P than the other materials at all time-points (P